Koller Antonius, Patel Purvi, Kim Jenny Kim, Chen Emily I
Herbert Irving Comprehensive Cancer Center, Proteomics Shared Resource, Columbia University Medical Center, New York, NY, 10032, USA.
Department of Pharmacology, Columbia University Medical Center, New York, NY, 10032, USA.
Methods Mol Biol. 2017;1619:213-225. doi: 10.1007/978-1-4939-7057-5_17.
Proteomics characterization of biofluids, such as urine and plasma, has been explored for the discovery of predictive, prognostic, and mechanistic biomarkers of diseases and tissue injury. Here we describe comprehensive characterization of protein cargos from cell-derived secreted vesicles (extracellular vesicles or exosome) for biomarker discovery using the mass spectrometry-based technology.
为了发现疾病和组织损伤的预测性、预后性及机制性生物标志物，人们已对尿液和血浆等生物流体进行蛋白质组学表征研究。在此，我们描述了利用基于质谱的技术对细胞衍生分泌囊泡（细胞外囊泡或外泌体）中的蛋白质成分进行全面表征以发现生物标志物的过程。
