支气管肺泡灌洗：基于定量质谱的蛋白质组学分析在肺部疾病中的应用

Bronchoalveolar Lavage: Quantitative Mass Spectrometry-Based Proteomics Analysis in Lung Diseases.

作者信息

Carvalho Ana Sofia, Matthiesen Rune

机构信息

Computational and Experimental Biology Group, CEDOC-Chronic Diseases Research Center, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Rua Câmara Pestana nº 6, 6-A, Lisboa, 1150-082, Portugal.

出版信息

Methods Mol Biol. 2017;1619:487-494. doi: 10.1007/978-1-4939-7057-5_34.

DOI:10.1007/978-1-4939-7057-5_34

PMID:28674906

Abstract

Bronchoalveolar lavage (BAL) fluid, obtained by a relatively noninvasive procedure, is used as a practice for diagnosis of various lung diseases as source of cells for cytology analysis. The acellular component of BAL potentially can complement and be a key for the establishment of diagnostic or as a prognostic indicator. This chapter discusses the aspects of standardization of BAL sample preparation and processing and its implications on the BAL fluid proteome quantitative analysis by high-throughput mass spectrometry. The detailed conditions for quantitative analysis of BAL proteome in the context of biomarker discovery are introduced.

摘要

支气管肺泡灌洗（BAL）液通过相对非侵入性的操作获取，作为各种肺部疾病诊断的一种手段，是用于细胞学分析的细胞来源。BAL的无细胞成分有可能起到补充作用，是建立诊断或作为预后指标的关键。本章讨论了BAL样本制备和处理的标准化方面及其对通过高通量质谱进行BAL液蛋白质组定量分析的影响。介绍了在生物标志物发现背景下BAL蛋白质组定量分析的详细条件。