Reisdorph Nichole A, Michel Cole, Fritz Kristofer, Reisdorph Richard
Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Methods Mol Biol. 2018;1809:237-262. doi: 10.1007/978-1-4939-8570-8_16.
Proteomics has enabled researchers to evaluate global protein changes in a relatively rapid and comprehensive manner. Applications of these technologies in lung research include biomarker and drug discovery, elucidating disease mechanisms, and quantitative clinical assays. Two common workflows exist for quantitative proteomics studies that are aimed at determining differences in protein levels: label-free and labeling methods. Here we describe specific techniques involved in both quantitative workflows; these include extensive sample preparation methods for several lung-specific sample types. Methods are also included for mass spectrometry-based sample analysis and data analysis. While the focus is on quantitative, clinical proteomics, these strategies are appropriate for a wide array of sample types and applications.
蛋白质组学使研究人员能够以相对快速和全面的方式评估全球蛋白质变化。这些技术在肺部研究中的应用包括生物标志物和药物发现、阐明疾病机制以及定量临床检测。针对确定蛋白质水平差异的定量蛋白质组学研究存在两种常见的工作流程：无标记法和标记法。在此，我们描述了这两种定量工作流程中涉及的具体技术；这些技术包括针对几种肺部特异性样本类型的广泛样本制备方法。还包括基于质谱的样本分析和数据分析方法。虽然重点是定量临床蛋白质组学，但这些策略适用于广泛的样本类型和应用。
