Department of Environmental and Occupational Health Sciences, University of Washington, 4225 Roosevelt Way NE, Suite 100, Seattle, WA, 98195, USA.
PATH, 2201 Westlake Ave., Suite 200, Seattle, WA, 98121, USA.
Food Environ Virol. 2018 Mar;10(1):72-82. doi: 10.1007/s12560-017-9311-7. Epub 2017 Jul 3.
Environmental surveillance of poliovirus (PV) plays an important role in the global program for eradication of wild PV. The bag-mediated filtration system (BMFS) was first developed in 2014 and enhances PV surveillance when compared to the two-phase grab method currently recommended by the World Health Organization (WHO). In this study, the BMFS design was improved and tested for its usability in wastewater and wastewater-impacted surface waters in Nairobi, Kenya. Modifications made to the BMFS included the size, color, and shape of the collection bags, the filter housing used, and the device used to elute the samples from the filters. The modified BMFS concentrated 3-10 L down to 10 mL, which resulted in an effective volume assayed (900-3000 mL) that was 6-20 times greater than the effective volume assayed for samples processed by the WHO algorithm (150 mL). The system developed allows for sampling and in-field virus concentration, followed by transportation of the filter for further analysis with simpler logistics than the current methods. This may ultimately reduce the likelihood of false-negative samples by increasing the effective volume assayed compared to samples processed by the WHO algorithm, making the BMFS a valuable sampling system for wastewater and wastewater-impacted surface waters.
环境监测在全球消灭野生脊灰病毒（PV）计划中发挥着重要作用。袋介导过滤系统（BMFS）于 2014 年首次开发，与世界卫生组织（WHO）目前推荐的两阶段抓取法相比，提高了 PV 监测能力。在这项研究中，对 BMFS 进行了改进，并在肯尼亚内罗毕的废水和受废水影响的地表水进行了可用性测试。对 BMFS 的修改包括收集袋的尺寸、颜色和形状、使用的过滤外壳以及用于从过滤器中洗脱样品的设备。改良后的 BMFS 将 3-10 L 浓缩至 10 mL，浓缩效率（900-3000 mL）比世卫组织算法处理的样品（150 mL）高 6-20 倍。该系统可用于采样和现场病毒浓缩，然后运输过滤器进行进一步分析，与现有方法相比，简化了物流。与世卫组织算法处理的样品相比，该系统通过增加浓缩效率，最终可能减少假阴性样本的可能性，使 BMFS 成为废水和受废水影响的地表水的有价值的采样系统。
