State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
Cell Stress Chaperones. 2018 Jan;23(1):13-28. doi: 10.1007/s12192-017-0820-y. Epub 2017 Jul 3.
Cyclin-dependent kinase 5 (CDK5) is an unusual CDK whose function has been implicated in protecting the central nervous system (CNS) from oxidative damage. However, there have been few studies of CDK5 in insects. In this study, we identified the AccCDK5 gene from Apis cerana cerana and investigated its role in oxidation resistance. We found that AccCDK5 is highly conserved across species and contains conserved features of the CDK5 family. The results of qPCR analysis indicated that AccCDK5 is highly expressed during the larval and pupal stages and in the adult head and muscle. We further observed that AccCDK5 is induced by several environmental oxidative stresses. Moreover, the overexpression of the AccCDK5 protein in E. coli enhances the resistance of the bacteria to oxidative stress. The activation of CDK5 requires binding to its activator. Therefore, we also identified and cloned cyclin-dependent kinase 5 regulatory subunit 1, which we named AccCDK5r1, from Apis cerana cerana. AccCDK5r1 contains a conserved cell localization targeting domain as well as binding and activation sites for CDK5. Yeast two-hybrid analysis demonstrated the interaction between AccCDK5 and AccCDK5r1. The expression patterns of the two genes were similar after stress treatment. Collectively, these results suggest that AccCDK5 plays a pivotal role in the response to oxidative stresses and that AccCDK5r1 is a potential activator of AccCDK5.
细胞周期蛋白依赖性激酶 5（CDK5）是一种不寻常的 CDK，其功能已被牵涉到保护中枢神经系统（CNS）免受氧化损伤。然而，关于昆虫中的 CDK5 的研究较少。在这项研究中，我们从中华蜜蜂中鉴定出 AccCDK5 基因，并研究了其在抗氧化中的作用。我们发现 AccCDK5 在物种间高度保守，并且含有 CDK5 家族的保守特征。qPCR 分析的结果表明，AccCDK5 在幼虫和蛹期以及成虫头部和肌肉中高度表达。我们进一步观察到 AccCDK5 被几种环境氧化应激诱导。此外，AccCDK5 蛋白在大肠杆菌中的过表达增强了细菌对氧化应激的抗性。CDK5 的激活需要与其激活剂结合。因此，我们还从中华蜜蜂中鉴定并克隆了细胞周期蛋白依赖性激酶 5 调节亚基 1，我们将其命名为 AccCDK5r1。AccCDK5r1 含有保守的细胞定位靶向结构域以及与 CDK5 结合和激活的位点。酵母双杂交分析表明 AccCDK5 与 AccCDK5r1 之间存在相互作用。在应激处理后，这两个基因的表达模式相似。总之，这些结果表明 AccCDK5 在应对氧化应激中发挥着关键作用，并且 AccCDK5r1 是 AccCDK5 的潜在激活剂。
