State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Cell Stress Chaperones. 2019 Nov;24(6):1137-1149. doi: 10.1007/s12192-019-01036-5. Epub 2019 Oct 29.
MAP kinase phosphatase 3 (MKP3), a member of the dual-specificity protein phosphatase (DUSP) superfamily, has been widely studied for its role in development, cancer, and environmental stress in many organisms. However, the functions of MKP3 in various insects have not been well studied, including honeybees. In this study, we isolated an MKP3 gene from Apis cerana cerana and explored the role of this gene in the resistance to oxidation. We found that AccMKP3 is highly conserved in different species and shares the closest evolutionary relationship with AmMKP3. We determined the expression patterns of AccMKP3 under various stresses. qRT-PCR results showed that AccMKP3 was highly expressed during the pupal stages and in adult muscles. We further found that AccMKP3 was induced in all the stress treatments. Moreover, we discovered that the enzymatic activities of peroxidase, superoxide dismutase, and catalase increased and that the expression levels of several antioxidant genes were affected after AccMKP3 was knocked down. Collectively, these results suggest that AccMKP3 may be associated with antioxidant processes involved in response to various environmental stresses.
丝氨酸/苏氨酸蛋白磷酸酶 3（MAP kinase phosphatase 3，MKP3）是双特异性蛋白磷酸酶（DUSP）超家族的成员，其在许多生物的发育、癌症和环境应激中的作用已被广泛研究。然而，MKP3 在各种昆虫中的功能尚未得到很好的研究，包括蜜蜂。在这项研究中，我们从中华蜜蜂（Apis cerana cerana）中分离出一个 MKP3 基因，并探索了该基因在氧化抗性中的作用。我们发现 AccMKP3 在不同物种中高度保守，与 AmMKP3 具有最接近的进化关系。我们确定了 AccMKP3 在各种应激下的表达模式。qRT-PCR 结果表明，AccMKP3 在蛹期和成虫肌肉中高度表达。我们进一步发现，AccMKP3 在所有应激处理中均被诱导。此外，我们发现过氧化物酶、超氧化物歧化酶和过氧化氢酶的酶活性增加，并且在 AccMKP3 被敲低后，几种抗氧化基因的表达水平受到影响。综上所述，这些结果表明 AccMKP3 可能与参与应对各种环境应激的抗氧化过程有关。
