生长抑素与受体结合的钙依赖性。

Calcium-dependence of somatostatin binding to receptors.

作者信息

Susini C, Esteve J P, Vaysse N, Ribet A

出版信息

Peptides. 1985 Sep-Oct;6(5):831-3. doi: 10.1016/0196-9781(85)90309-2.

Abstract

Binding of 125I-[Tyr11] somatostatin (S14) and 125I-[Tyr1]S14 has been studied in pancreatic acini and cerebral cortex. Ca2+-dependence of somatostatin binding to receptors was observed only with the highly non degradable iodinated analog 125I-[Tyr11] somatostatin but not with 125I-[Tyr1] somatostatin. The inhibitory activity of S14 on secretin-stimulated cAMP cellular content was decreased when Ca2+ concentration in the medium was reduced to 30 nM, indicating that the Ca2+ dependence of somatostatin binding to receptors is also present with the native peptide.

摘要

已在胰腺腺泡和大脑皮层中研究了¹²⁵I-[酪氨酸¹¹]生长抑素（S14）和¹²⁵I-[酪氨酸¹]S14的结合情况。仅在高度不可降解的碘化类似物¹²⁵I-[酪氨酸¹¹]生长抑素中观察到生长抑素与受体结合的钙依赖性，而¹²⁵I-[酪氨酸¹]生长抑素则未观察到。当培养基中的钙离子浓度降至30 nM时，S14对促胰液素刺激的细胞内环磷酸腺苷（cAMP）含量的抑制活性降低，这表明天然肽也存在生长抑素与受体结合的钙依赖性。