Baldino F, Wolfson B
Prog Clin Biol Res. 1985;192:409-21.
Considerable evidence has accumulated, suggesting that neurotensin (NT) interacts with neurotransmitter systems in the CNS. The purpose of this study was to investigate possible neurophysiological interactions between NT and putative neurotransmitters endogenous to the preoptic anterior hypothalamus (POAH). Using standard in vitro recording techniques, extracellular single unit activity was monitored in explant tissue cultures prepared from the POAH of newborn rats. Drugs were applied through the superfusion system or inotophoretically or pneumatically through micropipettes. Neurotensin produced a potent excitatory effect which enhanced lutamate induced excitations. In contrast, neurotensin reduced the effects of acetylcholine and had no effect on the actions of GABA and dopamine. These results suggest that interactions between NT and putative neurotransmitters in the POAH are highly specific.
大量证据不断积累，表明神经降压素（NT）与中枢神经系统中的神经递质系统相互作用。本研究的目的是调查NT与视前区下丘脑前部（POAH）内源性假定神经递质之间可能存在的神经生理相互作用。使用标准的体外记录技术，在新生大鼠POAH制备的外植体组织培养物中监测细胞外单单位活动。药物通过灌流系统、离子电泳或通过微量移液器以气动方式施加。神经降压素产生了强大的兴奋作用，增强了谷氨酸诱导的兴奋。相比之下，神经降压素减弱了乙酰胆碱的作用，对GABA和多巴胺的作用没有影响。这些结果表明，POAH中NT与假定神经递质之间的相互作用具有高度特异性。
