Baldino F, Higgins G A, Moke M T, Wolfson B
Peptides. 1985 Mar-Apr;6(2):249-56. doi: 10.1016/0196-9781(85)90048-8.
Anatomical and electrophysiological studies with neurotensin and somatostatin were performed to assess the integrity of primary explants of the preoptic anterior hypothalamus (POAH) maintained in organ culture as a model to study peptidergic neurons in vitro. POAH neurons maintained in vitro were found to be morphologically similar to those visualized histologically in situ. Somatostatin- and neurotensin-immunoreactive neurons were also present in both preparations. Additionally, electrophysiological responses of neurons in vitro to neurotensin and somatostatin were qualitatively similar to neuronal responses in situ. The striking morphological and pharmacological similarity between POAH neurons in vitro and in situ suggests that primary explants of the POAH are organotypic.
利用神经降压素和生长抑素进行了解剖学和电生理学研究，以评估视前区下丘脑前部（POAH）原代外植体在器官培养中的完整性，该外植体作为体外研究肽能神经元的模型。发现体外培养的POAH神经元在形态上与原位组织学观察到的神经元相似。生长抑素和神经降压素免疫反应性神经元在两种制剂中均有存在。此外，体外培养的神经元对神经降压素和生长抑素的电生理反应在性质上与原位神经元反应相似。体外和原位POAH神经元之间显著的形态学和药理学相似性表明，POAH原代外植体是器官型的。
