Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP12602-810, Brazil.
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP12602-810, Brazil.
Bioresour Technol. 2017 Nov;243:264-272. doi: 10.1016/j.biortech.2017.06.088. Epub 2017 Jun 19.
Surfactants are amphiphilic molecules with large industrial applications produced currently by chemical routes mainly derived from oil industry. However, biotechnological process, aimed to develop new sustainable process configurations by using favorable microorganisms, already requires investigations in more details. Thus, we present a novel approach for biosurfactant production using the promising yeast Aureobasidium pullulans LB 83, in stirred tank reactor. A central composite face-centered design was carried out to evaluate the effect of the aeration rate (0.1-1.1min) and sucrose concentration (20-80g.L) in the biosurfactant maximum tensoactivity and productivity. Statistical analysis showed that the use of variables at high levels enhanced tensoactivity, showing 8.05cm in the oil spread test and productivity of 0.0838cm.h. Also, unprecedented investigation of aeration rate and sucrose concentration relevance in biosurfactant production by A. pullulans in stirred tank reactor was detailed, demonstrating the importance to establish adequate conditions in bioreactors, aimed to scale-up process.
表面活性剂是具有大工业应用的两亲性分子，目前主要通过源自石油工业的化学途径生产。然而，生物技术过程旨在通过使用有利的微生物开发新的可持续工艺配置，已经需要更详细的研究。因此，我们提出了一种使用有前途的酵母出芽短梗霉 LB83 在搅拌釜式反应器中生产生物表面活性剂的新方法。进行了中心复合面心设计，以评估通气速率（0.1-1.1min）和蔗糖浓度（20-80g.L）对生物表面活性剂最大张力活性和生产力的影响。统计分析表明，在高变量水平下使用变量可以增强张力活性，在油扩散试验中显示出 8.05cm 的张力活性和 0.0838cm.h 的生产力。此外，还详细研究了搅拌釜式反应器中出芽短梗霉生产生物表面活性剂时通气速率和蔗糖浓度的相关性，这表明在生物反应器中建立适当的条件以实现工艺放大非常重要。
