Food Engineering and Technology Department, Institute of Chemical Technology (Deemed University), Matunga, Mumbai, India.
Bioprocess Biosyst Eng. 2013 Aug;36(8):1063-70. doi: 10.1007/s00449-012-0859-1. Epub 2012 Nov 24.
Scale up studies for production of lipoic acid (LA) from Saccharomyces cerevisiae have been reported in this paper for the first time. LA production in batch mode was carried out in a stirred tank bioreactor at varying agitation and aeration with maximum LA production of 512 mg/L obtained at 350 rpm and 25 % dissolved oxygen in batch culture conditions. Thus, LA production increased from 352 mg/L in shake flask to 512 mg/L in batch mode in a 5 L stirred tank bioreactor. Biomass production under these conditions was mathematically explained using logistic equation and data obtained for LA production and substrate utilization were successfully fitted using Luedeking-Piret and Mercier's models. The kinetic studies showed LA production to be growth associated. Further enhancement of LA production was carried out using fed-batch (variable volume) and semi-continuous modes of fermentation. Semi-continuous fermentation with three feeding cycles of sucrose effectively increased the production of LA from 512 to 725 mg/L.
本文首次报道了从酿酒酵母中大规模生产硫辛酸（LA）的研究。在搅拌罐生物反应器中分批进行 LA 生产，在不同的搅拌和通气条件下进行，在分批培养条件下，最大 LA 产量为 350rpm 和 25%溶解氧时的 512mg/L。因此，LA 的产量从摇瓶中的 352mg/L 增加到 5L 搅拌罐生物反应器中的 512mg/L。使用逻辑方程对这些条件下的生物量生产进行了数学解释，并且成功地使用 Luedeking-Piret 和 Mercier 模型拟合了 LA 生产和基质利用的数据。动力学研究表明，LA 的生产与生长有关。使用分批（变体积）和半连续发酵模式进一步提高了 LA 的产量。通过三次蔗糖补料循环的半连续发酵有效地将 LA 的产量从 512mg/L 提高到 725mg/L。
