Department of Dermatology, Zealand University Hospital, Roskilde, Denmark.
Dermatology. 2017;233(2-3):126-128. doi: 10.1159/000475925. Epub 2017 Jul 4.
Hidradenitis suppurativa is a skin disease of recurrent episodes of inflammatory nodules, abscesses, and scarring of the intertriginous regions, e.g. the axillae and groin. A dysregulated immune response to one or more unknown antigens in hidradenitis suppurativa has been suggested. One hypothetical element of this dysregulation may be the functionality of the cytokines. This study examines the serum level of anticytokine autoantibodies for interleukin (IL)-1α, IL-6, IL-10, IL-17A, IL-17E, IL-17F, and interferon-α.
Recombinant, carrier-free cytokines were coupled to microspheres. The coupled beads were incubated for 1 h in the dark with assay buffer-diluted sera, and subsequently for 30 min with polyclonal goat F(ab')2 anti-human IgG phycoerythrin-conjugated antibody. Data are presented as the median fluorescence intensity of samples.
No difference in levels of anticytokine autoantibodies was demonstrated for any of the autoantibodies studied.
The data suggest that endogenously produced autoantibodies only play a minor role, if any, in hidradenitis suppurativa.
化脓性汗腺炎是一种反复发作的炎症性结节、脓肿和皱褶部位（如腋窝和腹股沟）瘢痕的皮肤病。化脓性汗腺炎的免疫反应失调可能与一种或多种未知抗原有关。这种失调的一个假设因素可能是细胞因子的功能。本研究检测了白细胞介素（IL）-1α、IL-6、IL-10、IL-17A、IL-17E、IL-17F 和干扰素-α的抗细胞因子自身抗体的血清水平。
将无载体的重组细胞因子偶联到微球上。将偶联的珠子在黑暗中与含测定缓冲液的血清孵育 1 小时，然后用多克隆山羊 F(ab')2 抗人 IgG 藻红蛋白结合物孵育 30 分钟。数据以样品的中荧光强度表示。
在所研究的任何自身抗体中，均未显示抗细胞因子自身抗体水平存在差异。
数据表明，内源性产生的自身抗体在化脓性汗腺炎中仅起次要作用（如果有作用的话）。
