González-Stegmaier Roxana, Villarroel-Espíndola Franz, Manríquez René, López Mauricio, Monrás Mónica, Figueroa Jaime, Enríquez Ricardo, Romero Alex
Instituto de Patología Animal, Universidad Austral de Chile, Valdivia, Chile; Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile; Centro FONDAP: Interdisciplinary Center for Aquaculture Research (INCAR), Chile.
Department of Pathology, School of Medicine, Yale University, New Haven, CT, USA.
Dev Comp Immunol. 2017 Nov;76:303-309. doi: 10.1016/j.dci.2017.06.018. Epub 2017 Jul 1.
Neuropeptide Y (NPY) plays different roles in mammals such as: regulate food intake, memory retention, cardiovascular functions, and anxiety. It has also been shown in the modulation of chemotaxis, T lymphocyte differentiation, and leukocyte migration. In fish, NPY expression and functions have been studied but its immunomodulatory role remains undescribed. This study confirmed the expression and synthesis of NPY in S. salar under inflammation, and validated a commercial antibody for NPY detection in teleost. Additionally, immunomodulatory effects of NPY were assayed in vitro and in vivo. Phagocytosis and superoxide anion production in leukocytes and SHK cells were induced under stimulation with a synthetic peptide. IL-8 mRNA was selectively and strongly induced in the spleen, head kidney, and isolated cells, after in vivo challenge with NPY. All together suggest that NPY is expressed in immune tissues and modulates the immune response in teleost fish.
神经肽Y（NPY）在哺乳动物中发挥着不同的作用，如：调节食物摄入、记忆保持、心血管功能和焦虑。它还在趋化作用、T淋巴细胞分化和白细胞迁移的调节中发挥作用。在鱼类中，已经对NPY的表达和功能进行了研究，但其免疫调节作用仍未得到描述。本研究证实了炎症条件下大西洋鲑中NPY的表达和合成，并验证了一种用于硬骨鱼中NPY检测的商业抗体。此外，还在体外和体内检测了NPY的免疫调节作用。在用合成肽刺激后，白细胞和SHK细胞中的吞噬作用和超氧阴离子产生被诱导。在用NPY进行体内攻击后，脾脏、头肾和分离细胞中IL-8 mRNA被选择性地强烈诱导。所有这些都表明NPY在免疫组织中表达，并调节硬骨鱼的免疫反应。
