Laboratorio de Parasitología, Hospital Nacional de Clínicas, Universidad Nacional de Córdoba, Argentina.
Laboratorio de Parasitología, Hospital Nacional de Clínicas, Universidad Nacional de Córdoba, Argentina.
Infect Genet Evol. 2017 Oct;54:170-175. doi: 10.1016/j.meegid.2017.06.031. Epub 2017 Jul 1.
In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection.
在这项研究中，我们描述了在眼科就诊的患者中棘阿米巴角膜炎（AK）的频率，并确定了自由生活阿米巴（FLA）分离株的种/基因型。通过形态学和分子特征对角膜刮片（CS）和隐形眼镜（CL）佩戴者的 FLA 进行了研究。使用社会人口统计学、临床发现和 CL 使用史等变量构建了一个数据库。在 2000 年 1 月至 2016 年 9 月期间，阿根廷科尔多瓦市大学医院眼科就诊的角膜病变患者被纳入研究。在分析的 CS 和 CL 中，分别检测到 1.5%（11/739）和 17%（11/65）的 FLA。从 CL 用户中分离出的 FLA 有 80.9%存在隐形眼镜维护不当的情况，4.8%（1/21）不是接触过户外水源的隐形眼镜用户，14.3%（3/21）没有关于隐形眼镜用户的数据。CS 和 LC 中分别有 100%和 82%的样本被证实存在棘阿米巴。与 AK 相关的最常见症状是眼红和畏光。CS 中的 FLA 属于 II 组，但 CL 中的 82%（9/11）和 18%（2/11）分别属于 II 组和 III 组。在 100%的棘阿米巴分离株中检测到 T4 基因型和 A. polyphaga 种。不良的 CL 卫生习惯突出表明，需要加强对 AK 的严重性和不当 CL 卫生习惯后果的教育。在 CS 和 CL 样本中均检测到 100%的基因型 T4，这与先前的发现一致，表明该基因型是迄今为止从眼部感染中分离出的最普遍的基因型。
