Hayashi Y, Yanagawa T, Azuma M, Yura Y, Yoshida H, Sato M
Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;50(1):1-11. doi: 10.1007/BF02889886.
The adenocarcinoma produced by transplantation into nude mice of a neoplastic human salivary intercalated duct cell line was treated with 0.1 ml of minimal essential medium (MEM) containing dibutyryl cyclic AMP (dB-cAMP) at a final concentration of 1 mM daily for 28 days and examined morphologically and immunohistochemically. The dB-cAMP treatment resulted in a marked suppression of tumor growth. In addition, tumor nests with a myoepithelial cell phenotype characterized by the presence of microfilament systems reactive with antimyosin and anti-S-100 protein sera were often observed in the treated tumors, but not in untreated controls. These findings lead us to suggest that neoplastic intercalated duct cells can be induced to differentiate into myoepithelial cells and that levels of cAMP within the cells may regulate this cytodifferentiation.
将人涎腺闰管癌细胞系移植到裸鼠体内产生的腺癌，每日用0.1 ml含二丁酰环磷腺苷（dB-cAMP）、终浓度为1 mM的最低必需培养基（MEM）处理28天，并进行形态学和免疫组织化学检查。dB-cAMP处理导致肿瘤生长明显受抑。此外，在经处理的肿瘤中常可见具有肌上皮细胞表型的肿瘤巢，其特征为存在与抗肌球蛋白和抗S-100蛋白血清反应的微丝系统，而在未处理的对照中则未观察到。这些发现提示，肿瘤性闰管细胞可被诱导分化为肌上皮细胞，且细胞内的cAMP水平可能调节这种细胞分化。
