Kawamata H, Azuma M, Yanagawa T, Yura Y, Yoshida H, Furumoto N, Sato M
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Cancer Invest. 1992;10(2):111-27. doi: 10.3109/07357909209032772.
We have found the emergence of myoepithelial cells (HSG-AZA1) in neoplastic human salivary intercalated duct cell line HSG in culture after treatment with 5-azacytidine. When HSG-ZAZ1 cells were cultured in the presence of N6,O2-dibutyryl cyclic adenosine 3',5'-monophosphate (dB-cAMP), they formed long cytoplasmic processes which were densely packed with ample microfibrils in addition to microtubule bundles. The expression of neuron-specific enolase, synaptophysin, and catecholamine as well as neurofilaments in the treated HSG-AZA1 cells was found by the immunofluorescence staining technique, immunoblotting, immunoelectron microscopy, or catecholamine fluorescence. Both the anchorage-independent and anchorage-dependent growths of HSG-AZA1 cells were suppressed in the presence of dB-cAMP. After the removal of dB-cAMP from the culture, the treated cells returned rapidly to the phenotype and growth rate of the untreated cells. These findings indicate that reversible differentiation into the neuron-like cells of HSG-AZA1 cells occurs in growth medium containing dB-cAMP.
我们发现在用5-氮杂胞苷处理后，培养的人唾液闰管癌细胞系HSG中出现了肌上皮细胞（HSG-AZA1）。当HSG-ZAZ1细胞在N6,O2-二丁酰环磷腺苷（dB-cAMP）存在的情况下培养时，它们形成了长的细胞质突起，除了微管束外，这些突起还密集地排列着丰富的微原纤维。通过免疫荧光染色技术、免疫印迹、免疫电子显微镜或儿茶酚胺荧光法，在经处理的HSG-AZA1细胞中发现了神经元特异性烯醇化酶、突触素、儿茶酚胺以及神经丝的表达。在dB-cAMP存在的情况下，HSG-AZA1细胞的非贴壁依赖性生长和贴壁依赖性生长均受到抑制。从培养基中去除dB-cAMP后，经处理的细胞迅速恢复到未处理细胞的表型和生长速率。这些发现表明，在含有dB-cAMP的生长培养基中，HSG-AZA1细胞可发生可逆性分化为神经元样细胞。
