Táncos Zsuzsanna, Nemes Csilla, Varga Eszter, Bock István, Rungarunlert Sasitorn, Tharasanit Theerawat, Techakumphu Mongkol, Kobolák Julianna, Dinnyés András
Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary; BioTalentum Ltd., Gödöllő, Hungary.
BioTalentum Ltd., Gödöllő, Hungary.
Stem Cell Res. 2017 May;21:16-18. doi: 10.1016/j.scr.2017.03.010. Epub 2017 Mar 16.
Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. Furthermore, the spontaneous differentiation potential of the iPSC was also tested in vivo by teratoma assay. The iPSC line showed normal karyotype. The advantages of using RbiPSC are the easy access to primary material and the possibility to study the efficacy and safety of the iPSC-based therapies on a non-rodent animal model.
兔胚胎成纤维细胞（RbEF）（来自Hycole杂交兔胎儿）通过慢病毒递送自我沉默的hOKSM多顺反子载体进行重编程。通过多能性相关标志物的表达以及向三个胚层的体外自发分化，验证了新生成的RbiPSC的多能性。此外，还通过畸胎瘤试验在体内测试了iPSC的自发分化潜能。该iPSC系显示出正常的核型。使用RbiPSC的优点是易于获取原代材料，并且有可能在非啮齿动物模型上研究基于iPSC的疗法的有效性和安全性。
