Xu Wenting, Li Huajin, Peng Liangyue, Pu Liyu, Xiang Sijia, Li Yue, Tao Leiting, Liu Wenbin, Liu Jinhui, Xiao Yamei, Liu Shaojun
State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
College of Life Sciences, Hunan Normal University, Changsha, China.
Front Cell Dev Biol. 2022 Jan 20;9:817779. doi: 10.3389/fcell.2021.817779. eCollection 2021.
The technique of induced pluripotent stem cells has significant application value in breeding and preserving the genetic integrity of fish species. However, it is still unclear whether the chemically induced pluripotent stem cells can be induced from non-mammalian cells or not. In this article, we first verify that fibroblasts of fish can be chemically reprogrammed into pluripotent stem cells. These induced pluripotent stem-like cells possess features of colony morphology, expression of pluripotent marker genes, formation of embryoid bodies, teratoma formation, and the potential to differentiate into germ cell-like cells . Our findings will offer a new way to generate induced pluripotent stem cells in teleost fish and a unique opportunity to breed commercial fish and even save endangered fish species.
诱导多能干细胞技术在鱼类物种的育种和遗传完整性保存方面具有重要的应用价值。然而，化学诱导多能干细胞是否能从非哺乳动物细胞中诱导产生仍不清楚。在本文中，我们首先证实鱼类成纤维细胞可以被化学重编程为多能干细胞。这些诱导多能干细胞样细胞具有集落形态、多能性标记基因表达、胚状体形成、畸胎瘤形成以及分化为生殖细胞样细胞的潜力等特征。我们的研究结果将为硬骨鱼类诱导多能干细胞的产生提供一种新方法，并为商业鱼类育种乃至拯救濒危鱼类物种提供独特的机会。
