Iqbal Z, Koenig H
Biochem Biophys Res Commun. 1985 Dec 17;133(2):563-73. doi: 10.1016/0006-291x(85)90943-x.
High potassium (50 mM) depolarization induces a rapid (less than 15 sec) increase in the levels of the polyamines putrescine, spermidine and spermine and their rate-regulating synthetic enzyme ornithine decarboxylase in synaptosomes from rat cerebral cortex. The ornithine decarboxylase inhibitor alpha-difluoromethylornithine blocked the K+-stimulated increase in enzyme activity and polyamines and also suppressed the increase in 45Ca2+ influx and efflux and the Ca2+-dependent release of GABA and norepinephrine. Added putrescine attenuated or negated the effects of alpha-difluoromethylornithine. These results suggest that enhanced polyamine synthesis is required for potassium depolarized stimulation of synaptic function.
高钾（50 mM）去极化可使大鼠大脑皮层突触体中的多胺腐胺、亚精胺和精胺及其速率调节合成酶鸟氨酸脱羧酶水平迅速（少于15秒）升高。鸟氨酸脱羧酶抑制剂α-二氟甲基鸟氨酸可阻断钾离子刺激引起的酶活性和多胺增加，还可抑制45Ca2+流入和流出的增加以及GABA和去甲肾上腺素的钙依赖性释放。添加腐胺可减弱或消除α-二氟甲基鸟氨酸的作用。这些结果表明，增强的多胺合成是钾去极化刺激突触功能所必需的。
