Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100000, P.R. China.
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100000, P.R. China.
Oncol Rep. 2017 Aug;38(2):837-842. doi: 10.3892/or.2017.5768. Epub 2017 Jun 29.
Tetramethylpyrazine (TMP) has exhibited various anticancer effects. However, its ability to inhibit proliferation, migration, and invasion of prostate cancer (PCa) PC-3 cells is still unclear. In the present study, different concentrations of TMP were co-incubated with PC-3 cells. The pcDNA-FOXM1 plasmid was transfected into cells before treatment with 500 µg/l TMP. The proliferative, migratory and invasive abilities of PC-3 cells were tested by MTT assay, wound healing assay and colony formation assay. Western blotting was used to investigate the expression of FOXM1. We found that, compared with the control, the proliferative, migratory and invasive abilities of PC-3 cells were decreased after incubation with different concentrations of TMP (P<0.01). The expression of FOXM1 was decreased in TMP-treated PC-3 cells (P<0.01). In addition, overexpression of FOXM1 reversed TMP-mediated inhibition of proliferation, migration and invasion of PC-3 cells. We also found that TMP inhibited PCa growth in vivo in a dose-dependent manner. These results suggest that TMP inhibits PC-3 cell proliferation, migration and invasion by downregulation of FOXM1.
川芎嗪（TMP）具有多种抗癌作用。然而，其抑制前列腺癌（PCa）PC-3 细胞增殖、迁移和侵袭的能力尚不清楚。在本研究中，将不同浓度的 TMP 与 PC-3 细胞共同孵育。用 500μg/l TMP 处理前，将 pcDNA-FOXM1 质粒转染入细胞。通过 MTT 检测、划痕愈合检测和集落形成检测来测试 PC-3 细胞的增殖、迁移和侵袭能力。通过 Western blot 检测 FOXM1 的表达。我们发现，与对照组相比，用不同浓度的 TMP 孵育后，PC-3 细胞的增殖、迁移和侵袭能力均降低（P<0.01）。TMP 处理的 PC-3 细胞中 FOXM1 的表达降低（P<0.01）。此外，FOXM1 的过表达逆转了 TMP 介导的 PC-3 细胞增殖、迁移和侵袭的抑制作用。我们还发现 TMP 以剂量依赖的方式抑制体内的前列腺癌生长。这些结果表明，TMP 通过下调 FOXM1 抑制 PC-3 细胞的增殖、迁移和侵袭。
