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肌原纤维蛋白-橄榄油复合凝胶的流变学与微观结构:不同非肉类蛋白质作为乳化剂的影响

Rheology and microstructure of myofibrillar protein-olive oil composite gels: effect of different non-meat protein as emulsifier.

作者信息

Wu Mangang, Fei Litian, Zhuang Tao, Lei Shumin, Ge Qingfeng, Yu Hai, Wang Jiahao, Wang Yaosong

机构信息

College of Food Science and Engineering, Yangzhou University, Yangzhou, China.

College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing, China.

出版信息

J Sci Food Agric. 2018 Jan;98(2):799-806. doi: 10.1002/jsfa.8528. Epub 2017 Aug 23.

Abstract

BACKGROUND

Heat-induced composite gels were prepared with 20 g kg (2%) myofibrillar protein (MP) sol and 100 g kg (10%) olive oil pre-emulsified by MP or non-meat protein in 0.6 mol L NaCl, at pH 6.2. The effect of different non-meat protein (soy protein isolate, egg-white protein isolate and sodium caseinate) pre-emulsions on the rheological properties and microstructure of MP gel was evaluated.

RESULTS

Adding emulsion enhanced the gel strength of MP gel except for the soy protein isolate (SPI) as emulsifier group, but all emulsion group markedly improved (P < 0.05) the water-holding capacity and the storage modulus (G') of MP gels. SDS-PAGE show that some non-meat protein bands partially participated in the formation of MP composite gels with different kinds of emulsion added. Micrographs revealed that these emulsions made the gels become denser and more compact with subtle diverse effects.

CONCLUSION

Different meat or non-meat proteins as emulsifier have varied impacts on the rheology and microstructure of MP gels, indicating the potential and feasibility of these non-meat proteins as emulsifiers to modify the textural properties in comminuted meat products. © 2017 Society of Chemical Industry.

摘要

背景

在pH值为6.2的0.6mol/L氯化钠溶液中,用20g/kg(2%)肌原纤维蛋白(MP)溶胶和100g/kg(10%)经MP或非肉蛋白预乳化的橄榄油制备热诱导复合凝胶。评估了不同非肉蛋白(大豆分离蛋白、蛋清分离蛋白和酪蛋白酸钠)预乳液对MP凝胶流变学特性和微观结构的影响。

结果

添加乳液提高了MP凝胶的凝胶强度,但以大豆分离蛋白(SPI)作为乳化剂的组除外,不过所有乳液组均显著提高(P<0.05)了MP凝胶的保水能力和储能模量(G')。SDS-PAGE表明,添加不同种类乳液后,一些非肉蛋白条带部分参与了MP复合凝胶的形成。显微照片显示,这些乳液使凝胶变得更致密、更紧实,且效果略有不同。

结论

不同的肉蛋白或非肉蛋白作为乳化剂对MP凝胶的流变学和微观结构有不同影响,表明这些非肉蛋白作为乳化剂在改善碎肉制品质地特性方面具有潜力和可行性。©2017化学工业协会。

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