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鹰嘴豆分离蛋白对猪肉肌原纤维蛋白热诱导凝胶特性的影响。

Effect of chickpea (Cicer arietinum L.) protein isolate on the heat-induced gelation properties of pork myofibrillar protein.

机构信息

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, PR China.

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, PR China.

出版信息

J Sci Food Agric. 2021 Mar 30;101(5):2108-2116. doi: 10.1002/jsfa.10833. Epub 2020 Oct 17.

DOI:10.1002/jsfa.10833
PMID:32978960
Abstract

BACKGROUND

Heat-induced composite gels were prepared with 30 mg mL pork myofibrillar protein (MP) and chickpea protein isolate (CPI) (0, 3, 6, 9, 12, and 15 g kg ) in 0.6 mol L NaCl, at pH 7.0. The gel strength, water-holding capacity, rheological properties, and microstructure of MP-CPI composite gels were investigated.

RESULTS

Chickpea protein isolate improved (P < 0.05) gel strength and water-holding capacity of the MP composite gels. The rheological properties of MP-CPI composite gels were improved significantly by the addition of CPI. Meanwhile, the effects of CPI on the storage modulus of composite gels were positively correlated with the increased addition of CPI. Furthermore, according to low-field nuclear magnetic resonance (LF-NMR) results, the addition of CPI reduced the relaxation time of the composite gels and the relaxation peak area of free water, indicating that CPI could improve the water-holding capacity of MP-CPI composite gels. The microstructure of MP-CPI composite gels presented smaller and more uniform pores, which means that more water could be retained.

CONCLUSION

The addition of chickpea protein isolate improved the gel strength, water-holding capacity, rheological properties, and microstructure of MP gels, indicating that CPI could be a potential protein additive to improve the microstructure, texture, and functional quality of meat products. © 2020 Society of Chemical Industry.

摘要

背景

在 pH 值 7.0、0.6 mol/L NaCl 中,用 30mg/mL 的猪肉肌原纤维蛋白(MP)和鹰嘴豆分离蛋白(CPI)(0、3、6、9、12 和 15g/kg)制备了热诱导复合凝胶。研究了 MP-CPI 复合凝胶的凝胶强度、持水性、流变学特性和微观结构。

结果

鹰嘴豆分离蛋白(CPI)提高了(P<0.05)MP 复合凝胶的凝胶强度和持水性。CPI 的添加显著改善了 MP-CPI 复合凝胶的流变学特性。同时,CPI 对复合凝胶储能模量的影响与 CPI 增加呈正相关。此外,根据低场核磁共振(LF-NMR)结果,CPI 的添加降低了复合凝胶的弛豫时间和自由水的弛豫峰面积,表明 CPI 可以提高 MP-CPI 复合凝胶的持水能力。MP-CPI 复合凝胶的微观结构呈现出更小且更均匀的孔,这意味着可以保留更多的水。

结论

添加鹰嘴豆分离蛋白(CPI)提高了 MP 凝胶的凝胶强度、持水性、流变学特性和微观结构,表明 CPI 可以作为一种潜在的蛋白质添加剂,改善肉类产品的微观结构、质地和功能质量。 © 2020 英国化学学会。

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