可裂变的人工真核细胞模型。

A Fissionable Artificial Eukaryote-like Cell Model.

机构信息

State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology , 92 West Da-Zhi Street, Harbin 150001, China.

出版信息

J Am Chem Soc. 2017 Jul 26;139(29):9955-9960. doi: 10.1021/jacs.7b04009. Epub 2017 Jul 14.

DOI:10.1021/jacs.7b04009

PMID:28677973

Abstract

The use of artificial cells has attracted considerable attention in various fields from biotechnology to medicine. Here, we develop a cell-sized vesicle-in-vesicle (VIV) structure containing a separate inner vesicle (IV) that can be loaded with DNA. We use polymerase chain reaction (PCR) to successfully amplify the amount of DNA confined to the IV. Subsequent osmotic stress-induced fission of a mother VIV into two daughter VIVs successfully divides the IV content while keeping it confined to the IV of the daughter VIVs. The fission rate was estimated to be ∼20% quantified by fluorescence microscope. Our VIV structure represents a step forward toward construction of an advanced, fissionable cell model.

摘要

人工细胞在生物技术到医学等各个领域引起了相当大的关注。在这里，我们开发了一种细胞大小的囊泡-囊泡（VIV）结构，其中包含一个可以装载 DNA 的独立内囊泡（IV）。我们使用聚合酶链反应（PCR）成功地扩增了限制在 IV 中的 DNA 量。随后，通过渗透压胁迫诱导的母 VIV 分裂成两个子 VIV，成功地在将 IV 内容物限制在子 VIV 的 IV 内的同时将其进行了分割。通过荧光显微镜定量估计的裂变率约为 20%。我们的 VIV 结构代表着朝着构建先进的、可裂变的细胞模型迈出了一步。