Blackshear Charles Philip, Rector Michael Anthony, Chung Natalie Narie, Irizarry Dre Michael, Flacco John Stephen, Brett Elizabeth Anne, Momeni Arash, Lee Gordon Kwanlyp, Longaker Michael T, Wan Derrick C
Ann Plast Surg. 2018 Mar;80(3):293-296. doi: 10.1097/SAP.0000000000001183.
Studies evaluating fat grafting in mice have frequently used micro-computed tomography (micro-CT) as an accurate radiographic tool to measure longitudinal volume retention without killing the animal. Over the past decade, however, microultrasonography has emerged as an equally powerful preclinical imaging tool. Given their respective strengths in 3-dimensional reconstruction, there is no study to our knowledge that directly compares micro-CT with microultrasound in volumetric analysis. In this study, we compared the performance of micro-CT with microultrasound in the evaluation of adipose tissue graft volume in a murine model. Fifteen immunodeficient mice were given 200 μL of adipose tissue grafts. In vivo volumetric analysis of the grafts by micro-CT and microultrasound was conducted at discrete time points up to postoperative day 105. Three mice were killed at multiple time points, and explanted grafts were reimaged by CT and ultrasound, as mentioned previously. Analysis revealed that in vivo graft volumes measured by micro-CT do not differ significantly from those of microultrasound. Furthermore, both micro-CT and microultrasound were capable of accurately measuring fat grafts as in vivo volumes closely correlated with explanted volumes. Finally, ultrasound was found to yield improved soft tissue contrast compared with micro-CT. Therefore, either modality may be used, depending on experimental needs.
评估小鼠脂肪移植的研究经常使用微型计算机断层扫描（micro-CT）作为一种精确的影像学工具，以在不杀死动物的情况下测量纵向体积保留情况。然而，在过去十年中，微型超声成像已成为一种同样强大的临床前成像工具。鉴于它们在三维重建方面各自的优势，据我们所知，尚无研究在体积分析中直接比较微型计算机断层扫描与微型超声。在本研究中，我们比较了微型计算机断层扫描与微型超声在评估小鼠模型中脂肪组织移植物体积方面的性能。给15只免疫缺陷小鼠移植200μL脂肪组织移植物。在术后第105天之前的不同时间点，通过微型计算机断层扫描和微型超声对移植物进行体内体积分析。在多个时间点处死3只小鼠，并如前所述，对取出的移植物进行CT和超声再次成像。分析显示，微型计算机断层扫描测量的体内移植物体积与微型超声测量的体积无显著差异。此外，微型计算机断层扫描和微型超声都能够准确测量脂肪移植物，因为体内体积与取出的体积密切相关。最后，发现超声与微型计算机断层扫描相比，能产生更好的软组织对比度。因此，可根据实验需要选择任何一种方式。
