Trulson M E, Cannon M S, Faegg T S, Raese J D
Brain Res Bull. 1985 Dec;15(6):569-77. doi: 10.1016/0361-9230(85)90206-0.
Chronic administration of methamphetamine (20 mg/kg, IP, every 12 hours for 10 days) produced a large decrease in tyrosine hydroxylase staining axons and terminal boutons in the caudate nucleus in rats when examined 60 days following the final methamphetamine injection. This effect was quantitated using the Leitz Data Acquisition and Display System (DADS) revealing that there was a 74% decrease in tyrosine hydroxylase positive processes in the caudate nucleus. Furthermore, this treatment also produced a large decrease in the number of tyrosine hydroxylase positive staining neuronal perikarya in the pars compacta of the substantia nigra. This effect was also quantitative using the Leitz-(DADS) system, revealing a decrease of 89% in tyrosine hydroxylase positive material. These data demonstrate that chronic administration of methamphetamine produces a long-term loss of tyrosine hydroxylase enzyme in both the cell bodies of the substantia nigra and the nerve terminals in the caudate nucleus. Whether this effect is due to the degeneration of the neurons or some metabolic effect remains to be determined.
对大鼠进行甲基苯丙胺慢性给药（20毫克/千克，腹腔注射，每12小时一次，共10天），在最后一次注射甲基苯丙胺60天后检查发现，大鼠尾状核中酪氨酸羟化酶染色的轴突和终末小体大幅减少。使用徕卡数据采集与显示系统（DADS）对这种效应进行定量分析，结果显示尾状核中酪氨酸羟化酶阳性突起减少了74%。此外，这种处理还使黑质致密部中酪氨酸羟化酶阳性染色的神经元胞体数量大幅减少。使用徕卡（DADS）系统对这种效应进行定量分析，结果显示酪氨酸羟化酶阳性物质减少了89%。这些数据表明，甲基苯丙胺的慢性给药会导致黑质细胞体和尾状核神经末梢中的酪氨酸羟化酶长期丧失。这种效应是由于神经元变性还是某种代谢效应，仍有待确定。
