与硼氧烷衍生物对过氧化物酶抑制作用的分光光度动力学研究相比，电化学方法的优势。

Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative.

作者信息

Ostojić Jelena, Herenda Safija, Bešić Zerina, Miloš Mladen, Galić Borivoj

机构信息

Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina.

Faculty of Chemistry and Technology, University of Split, Teslina 10, 21 000 Split, Croatia.

出版信息

Molecules. 2017 Jul 5;22(7):1120. doi: 10.3390/molecules22071120.

In this study, boroxine derivative (K₂[B₃O₃F₄OH]) was tested as an inhibitor of horseradish peroxidase (HRP) by spectrophotometric and electrochemical methods. The activity of horseradish peroxidase was first studied under steady-state kinetic conditions by a spectrophotometric method which required the use of guaiacol as a second substrate to measure guaiacol peroxidation. The results of this method have shown that, by changing the concentration of guaiacol as the literature suggests, a different type of inhibition is observed than when changing the concentration of hydrogen peroxide as the substrate. This suggests that guaiacol interferes with the reaction in some way. The electrochemical method involves direct electron transfer of HRP immobilized in Nafion nanocomposite films on a glassy carbon (GC) electrode, creating a sensor with an electro-catalytic response to the reduction of hydrogen peroxide. The electrochemical method simplifies kinetic assays by removing the requirement of reducing substrates.

在本研究中，采用分光光度法和电化学方法测试了硼氧环衍生物（K₂[B₃O₃F₄OH]）作为辣根过氧化物酶（HRP）抑制剂的性能。首先，通过分光光度法在稳态动力学条件下研究辣根过氧化物酶的活性，该方法需要使用愈创木酚作为第二底物来测量愈创木酚的过氧化反应。该方法的结果表明，正如文献所建议的那样，通过改变愈创木酚的浓度，观察到的抑制类型与改变过氧化氢作为底物的浓度时不同。这表明愈创木酚以某种方式干扰了反应。电化学方法涉及固定在玻碳（GC）电极上的Nafion纳米复合膜中的HRP的直接电子转移，从而创建了一种对过氧化氢还原具有电催化响应的传感器。电化学方法通过消除对还原底物的需求简化了动力学测定。