a Laboratorio de Señalización Intracelular, Sección de Posgrado , Escuela Superior de Medicina del Instituto Politécnico Nacional Ciudad de México , México.
b CIASaP, Facultad de Medicina , Universidad Autónoma de Sinaloa Culiacán , Sinaloa , México.
Clin Exp Hypertens. 2017;39(6):513-519. doi: 10.1080/10641963.2016.1273949. Epub 2017 Jul 5.
Hypertension (HBP) is a chronic disease characterized by increased blood pressure, which despite several treatments maintains a high morbi-mortality, which suggests that there are other mechanisms involved in this pathology, within which the orphan receptors could be candidates for the treatment of the HBP; these receptors are called orphan receptors because their ligand is unknown. These receptors have been suggested to participate in some pathologies because they are associated with various systems such as GPR88, which has been linked to the dopaminergic system, and GPR124 with angiogenesis, suggesting that these receptors could take part in HBP. Hence, the aim of this work was to study the expression of orphan receptors GPR88 and GPR124 in various tissues of normotensive and hypertensive rats. We used Wistar Kyoto (WKY) and spontaneously hypertensive rat (SHR) of 6-8 and 10-12 weeks of age and we determined systolic blood pressure (SBP), heart rate, as well as mRNA of GPR88 and GPR124 receptors by reverse transcription polymerase chain reaction (RT-PCR) in the aorta, heart, kidney, and brain. Our results showed that GPR88 and GPR124 were expressed in all analyzed tissues, but their expression is dependent on the age and development of HBP because their expression tends to be modified as HBP is established. Therefore, we conclude that GPR88 and GPR124 receptors may be involved in the development or maintenance of high blood pressure.
高血压（HBP）是一种以血压升高为特征的慢性疾病，尽管进行了多种治疗，但仍保持着较高的死亡率和发病率，这表明在这种病理中还存在其他机制，孤儿受体可能是治疗 HBP 的候选药物；这些受体之所以被称为孤儿受体，是因为它们的配体未知。这些受体被认为参与了一些病理学，因为它们与各种系统相关，如 GPR88，它与多巴胺能系统有关，而 GPR124 与血管生成有关，这表明这些受体可能参与 HBP。因此，这项工作的目的是研究孤儿受体 GPR88 和 GPR124 在正常血压和高血压大鼠的各种组织中的表达。我们使用了 6-8 周和 10-12 周龄的 Wistar Kyoto（WKY）和自发性高血压大鼠（SHR），并通过逆转录聚合酶链反应（RT-PCR）测定了主动脉、心脏、肾脏和大脑中的 GPR88 和 GPR124 受体的 mRNA 表达、收缩压（SBP）和心率。我们的结果表明，GPR88 和 GPR124 在所有分析的组织中均有表达，但它们的表达取决于 HBP 的年龄和发展，因为随着 HBP 的建立，其表达趋于改变。因此，我们得出结论，GPR88 和 GPR124 受体可能参与高血压的发展或维持。
