Department of Oral and Maxillofacial Pathology, School of Dentistry, Universidade de Pernambuco, Camaragibe, Pernambuco, Brazil.
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
J Endod. 2018 Dec;44(12):1783-1787. doi: 10.1016/j.joen.2018.09.002.
The purpose of this study was to evaluate the expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor alpha (TNF-α) in periapical granuloma (PG) and radicular cyst (RC) samples and to correlate it with the type of lesion, the intensity of the inflammatory infiltrate, and the thickness of the epithelial lining.
A total of 51 cases of periapical lesions (25 PGs and 26 RCs) were subjected to morphologic analysis and immunohistochemical study. The anti-COX-2 and anti-TNF-α antibodies were applied using the immunoperoxidase technique. Data were analyzed by the Mann-Whitney test, Pearson chi-square test, Fisher exact test, and Spearman correlation.
Analysis of the inflammatory infiltrate revealed that 80% of PGs exhibited a grade III infiltrate as opposed to a 19% rate in RCs (P < .001). Morphologic evaluation of the epithelial thickness of RCs revealed the presence of atrophic epithelium in 73% of cases. The majority of PGs had a score of 1 for COX-2 immunoexpression (n = 14, 54%) and a score of 2 for TNF-α expression (n = 16, 64%), whereas in cases of RCs a score of 1 was more prevalent for COX-2 and TNF-α expression (n = 17, 65%). Significant differences in the expression scores of COX-2 and TNF-α were detected in periapical lesions (P < .001).
Based on these findings, we emphasize that RCs and PGs have a similar expression of inflammatory mediators (COX-2 and TNF-α) although the secretion of TNF-α by macrophages and of COX-2 by several cells was higher in PGs, indicating a greater inflammatory response in these lesions.
本研究旨在评估环氧化酶-2（COX-2）和肿瘤坏死因子-α（TNF-α）在根尖肉芽肿（PG）和根侧囊肿（RC）样本中的表达，并将其与病变类型、炎症浸润强度和上皮衬里厚度相关联。
对 51 例根尖病变（25 例 PG 和 26 例 RC）进行形态学分析和免疫组织化学研究。采用免疫过氧化物酶技术应用抗 COX-2 和抗 TNF-α 抗体。采用 Mann-Whitney 检验、Pearson 卡方检验、Fisher 确切检验和 Spearman 相关分析进行数据分析。
对炎症浸润的分析表明，80%的 PG 表现为 III 级浸润，而 RC 为 19%（P<.001）。RC 上皮厚度的形态学评估显示，73%的病例存在萎缩性上皮。大多数 PG 的 COX-2 免疫表达评分为 1（n=14，54%），TNF-α 表达评分为 2（n=16，64%），而 RC 的 COX-2 和 TNF-α 表达评分更常见为 1（n=17，65%）。根尖病变中 COX-2 和 TNF-α 的表达评分存在显著差异（P<.001）。
根据这些发现，我们强调 RC 和 PG 具有相似的炎症介质（COX-2 和 TNF-α）表达，尽管巨噬细胞分泌 TNF-α和多种细胞分泌 COX-2 在 PG 中更高，表明这些病变中存在更大的炎症反应。
