Nassar C F, Nasser M G, Habbal Z M
Gen Pharmacol. 1985;16(6):625-7. doi: 10.1016/0306-3623(85)90155-7.
Prostaglandin E1 binding to isolated liver plasma membrane as a function of PGE1 concentration showed saturability of the binding sites at PGE1 concentration of 2.5 X 10(-4) M. Scatchard analysis revealed heterogeneous population of binding sites with a binding capacity of 470 and 990 nmol/mg protein for the higher and lower affinity binding sites respectively. PGE1 binding to liver plasma membrane was progressively and significantly decreased (P less than 0.01) as the incubation temperature was reduced to 22 degrees and 4 degrees C. Procaine at 1 X 10(-3) M concentration showed a significant decrease (P less than 0.01) in the binding capacity of the liver plasma membrane. Colchicine plus cytochalasin-B inhibited PGE1 binding significantly (P less than 0.01) but their inhibition is not equivalent to that of procaine.
前列腺素E1与分离的肝细胞膜结合情况随PGE1浓度的变化显示，在PGE1浓度为2.5×10⁻⁴ M时结合位点出现饱和。Scatchard分析表明存在异质性结合位点群体，高亲和力和低亲和力结合位点的结合容量分别为470和990 nmol/mg蛋白质。随着孵育温度降至22℃和4℃，PGE1与肝细胞膜的结合逐渐且显著降低（P<0.01）。1×10⁻³ M浓度的普鲁卡因使肝细胞膜的结合容量显著降低（P<0.01）。秋水仙碱加细胞松弛素B显著抑制PGE1结合（P<0.01），但其抑制作用与普鲁卡因不同。
