Matsumoto M, Kikuchi E, Okamoto Y, Morimura M, Kitano H, Fukui H, Nakano H, Tsujii T
Third Department of Internal Medicine, Nara Medical University, Japan.
Biochem Biophys Res Commun. 1992 Apr 30;184(2):654-60. doi: 10.1016/0006-291x(92)90639-3.
Specific bindings of [3H]prostaglandin E1 ([3H]PGE1), 125I-glucagon and [3H]norepinephrine to D-galactosamine (GalN)-treated rat hepatocytes in primary culture were investigated. After a two hour-treatment with GalN (1 and 10mg/ml), hepatocytes showed an enhanced specific binding to [3H]PGE1, whereas 125I-glucagon binding was little affected and [3H]norepinephrine binding was strongly diminished. Scatchard plot analysis indicated an increase of binding sites of [3H]PGE1. This unusual manner of [3H]PGE1 binding is suggested to indicate a special property of PGE1 receptor and may be associated with the cytoprotective effect of prostaglandins.
研究了[3H]前列腺素E1（[3H]PGE1）、125I-胰高血糖素和[3H]去甲肾上腺素与原代培养的经D-半乳糖胺（GalN）处理的大鼠肝细胞的特异性结合。在用GalN（1和10mg/ml）处理两小时后，肝细胞对[3H]PGE1的特异性结合增强，而125I-胰高血糖素结合几乎未受影响，[3H]去甲肾上腺素结合则显著减少。Scatchard作图分析表明[3H]PGE1结合位点增加。这种[3H]PGE1结合的异常方式提示PGE1受体具有特殊性质，可能与前列腺素的细胞保护作用有关。
