Frantz Eliete Dalla Corte, Giori Isabele Gomes, Machado Marcus Vinícius, Magliano D'Angelo Carlo, Freitas Fernanda Marques, Andrade Mariana Sodré Boêta, Vieira Aline Bomfim, Nóbrega Antonio Claudio Lucas, Tibiriçá Eduardo
Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niteroi, Brazil; and.
Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
Am J Physiol Endocrinol Metab. 2017 Oct 1;313(4):E473-E482. doi: 10.1152/ajpendo.00078.2017. Epub 2017 Jul 5.
Metabolic syndrome is a cluster of metabolic risk factors that is linked to central obesity, elevated blood pressure, insulin resistance (IR), and dyslipidemia, where the renin-angiotensin system (RAS) may provide a link among them. This study aimed to evaluate volume exercise effects comparing low vs. high volume of chronic aerobic exercise on RAS axes in skeletal muscle in a diet-induced obesity (DIO) rat model. For this, male Wistar-Kyoto rats were fed a standard chow (SC) diet or a high-fat (HF) diet for 32 wk. Animals receiving the HF diet were randomly divided into low exercise volume (LEV, 150 min/wk) and high exercise volume (HEV, 300 min/wk) at the 20th week. After 12 wk of aerobic treadmill training, the body mass and composition, blood pressure, glucose and lipid metabolism, RAS axes, insulin signaling, and inflammatory pathway were performed. HEV slowed the body mass gain, reduced intra-abdominal fat pad and leptin levels, improved total and peripheral body composition and inflammatory cytokine, reduced angiotensin II type 1 receptor expression, and increased Mas receptor protein expression compared with the HF animals. Sedentary groups (SC and HF) presented lower time to exhaustion and maximal velocity compared with the LEV and HEV groups. Both exercise training groups showed reduced resting systolic blood pressure and heart rate, improved glucose tolerance, IR, insulin signaling, and lipid profile. We conclude that the HEV, but not LEV, shifted the balance of RAS toward the ACE2/Mas receptor axis in skeletal muscle, presenting protective effects against the DIO model.
代谢综合征是一组与中心性肥胖、血压升高、胰岛素抵抗（IR）和血脂异常相关的代谢风险因素，其中肾素-血管紧张素系统（RAS）可能在它们之间起到联系作用。本研究旨在评估在饮食诱导肥胖（DIO）大鼠模型中，低运动量与高运动量的慢性有氧运动对骨骼肌中RAS轴的影响。为此，雄性Wistar-Kyoto大鼠被喂食标准饲料（SC）或高脂（HF）饲料32周。在第20周时，接受HF饮食的动物被随机分为低运动量组（LEV，150分钟/周）和高运动量组（HEV，300分钟/周）。经过12周的有氧跑步机训练后，对体重和组成、血压、糖脂代谢、RAS轴、胰岛素信号和炎症途径进行了检测。与HF组动物相比，HEV减缓了体重增加，减少了腹部脂肪垫和瘦素水平，改善了全身和外周身体组成以及炎症细胞因子，降低了血管紧张素II 1型受体表达，并增加了Mas受体蛋白表达。与LEV组和HEV组相比，久坐组（SC组和HF组）的力竭时间和最大速度较低。两个运动训练组均显示静息收缩压和心率降低，葡萄糖耐量、IR以及胰岛素信号和血脂状况得到改善。我们得出结论，高运动量（HEV）而非低运动量（LEV）使骨骼肌中RAS的平衡向ACE2/Mas受体轴转移，对DIO模型具有保护作用。
