Vecchiatto Bruno, da Silva Rafael C, Higa Talita S, Muller Cynthia R, Américo Anna Laura V, Fortunato-Lima Vanessa C, Ferreira Marília M, Martucci Luiz Felipe, Fonseca-Alaniz Miriam H, Evangelista Fabiana S
School of Arts, Science and Humanities, University of Sao Paulo, Av. Arlindo Bettio, 1000, Ermelino Mattarazzo, São Paulo, SP, CEP 03828-000, Brazil.
Department of Experimental Pathophysiology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Diabetol Metab Syndr. 2021 Jul 6;13(1):74. doi: 10.1186/s13098-021-00693-w.
We investigate the effect of aerobic physical training (APT) on muscle morphofunctional markers and Angiotensin Converting Enzyme 2/Angiotensin 1-7/Mas receptor (ACE2/Ang 1-7/Mas) axis in an obesity-linked insulin resistance (IR) animal model induced by cafeteria diet (CAF).
Male C57BL/6J mice were assigned into groups CHOW-SED (chow diet, sedentary; n = 10), CHOW-TR (chow diet, trained; n = 10), CAF-SED (n = 10) and CAF-TR (n = 10). APT consisted in running sessions of 60 min at 60% of maximal speed, 5 days per week for 8 weeks.
Trained groups had lower body weight and adiposity compared with sedentary groups. CAF-TR improved the glucose and insulin tolerance tests compared with CAF-SED group (AUC = 28.896 ± 1589 vs. 35.200 ± 1076 mg dL 120 min; kITT = 4.1 ± 0.27 vs. 2.5 ± 0.28% min, respectively). CHOW-TR and CAF-TR groups increased exercise tolerance, running intensity at which VO max was reached, the expression of p-AMPK, p-ACC and PGC1-α proteins compared with CHOW-SED and CAF-SED. Mithocondrial protein expression of Mfn1, Mfn2 and Drp1 did not change. Lipid deposition reduced in CAF-TR compared with CAF-SED group (3.71 vs. 5.53%/area), but fiber typing, glycogen content, ACE2 activity, Ang 1-7 concentration and Mas receptor expression did not change.
The APT prevents obesity-linked IR by modifying the skeletal muscle phenotype to one more oxidative independent of changes in the muscle ACE2/Ang 1-7/Mas axis.
我们研究了有氧体育锻炼（APT）对由自助餐饮食（CAF）诱导的肥胖相关胰岛素抵抗（IR）动物模型中肌肉形态功能标志物和血管紧张素转换酶2/血管紧张素1-7/ Mas受体（ACE2/Ang 1-7/Mas）轴的影响。
将雄性C57BL/6J小鼠分为CHOW-SED组（普通饮食，久坐不动；n = 10）、CHOW-TR组（普通饮食，训练有素；n = 10）、CAF-SED组（n = 10）和CAF-TR组（n = 10）。APT包括以最大速度的60%进行60分钟的跑步训练，每周5天，共8周。
与久坐组相比，训练组的体重和肥胖程度较低。与CAF-SED组相比，CAF-TR组改善了葡萄糖和胰岛素耐量试验（AUC分别为28.896±1589与35.200±1076 mg dL 120分钟；kITT分别为4.1±0.27与2.5±0.28%分钟）。与CHOW-SED组和CAF-SED组相比，CHOW-TR组和CAF-TR组提高了运动耐力、达到最大摄氧量时的跑步强度、p-AMPK、p-ACC和PGC1-α蛋白的表达。Mfn1、Mfn2和Drp1的线粒体蛋白表达没有变化。与CAF-SED组相比，CAF-TR组的脂质沉积减少（3.71%对5.53%/面积），但纤维类型、糖原含量、ACE2活性、Ang 1-7浓度和Mas受体表达没有变化。
APT通过将骨骼肌表型改变为更具氧化性的表型来预防肥胖相关的IR，而与肌肉ACE2/Ang 1-7/Mas轴的变化无关。
