在伊朗东北部患有肺部疾病的免疫功能低下患者中使用聚合酶链反应进行分子检测。
Molecular detection of using polymerase chain reaction in immunocompromised patients with pulmonary disorders in northeast of Iran.
作者信息
Parian M, Fata A, Najafzadeh M J, Rezaeitalab F
机构信息
Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Research Center for Skin Diseases & Cutaneous Leishmaniasis, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Curr Med Mycol. 2015 Jun;1(2):13-18. doi: 10.18869/acadpub.cmm.1.2.13.
BACKGROUND AND PURPOSE
Pneumocystis pneumonia, caused by , is a fatal disease threatening patients with AIDS or immunosuppression. Assessment of colonization in these patients is of great significance, since it can lead to severe pulmonary disorders. Considering the scarcity of published reports on isolates from patients in Mashhad, Iran, we aimed to evaluate pneumocystis colonization in individuals with different pulmonary disorders.
MATERIALS AND METHODS
We used nested polymerase chain reaction (PCR) method to amplify mitochondrial large subunit-ribosomal ribonucleic acid (mtLSU-rRNA) gene in 60 bronchoalveolar lavage (BAL) samples, obtained from patients, referring to the Department of Internal Medicine (Pulmonary Diseases Section) at Imam Reza Hospital, affiliated to Mashhad University of Medical Sciences, Mashhad, Iran.
RESULTS
DNA of was detected in 10 out of 60 BAL samples (16.66%) via nested PCR method.
CONCLUSION
According to the present findings, the colonization rate of was similar to the rates reported in other similar studies in Iran.
背景与目的
由[病原体名称未给出]引起的肺孢子菌肺炎是一种威胁艾滋病患者或免疫抑制患者生命的致命疾病。评估这些患者的感染情况具有重要意义，因为这可能导致严重的肺部疾病。鉴于伊朗马什哈德地区关于从患者身上分离出[病原体名称未给出]的已发表报告较少，我们旨在评估不同肺部疾病患者的肺孢子菌感染情况。
材料与方法
我们采用巢式聚合酶链反应（PCR）方法，对从伊朗马什哈德医科大学附属伊玛目礼萨医院内科（肺病科）获取的60份支气管肺泡灌洗（BAL）样本中的线粒体大亚基核糖体核糖核酸（mtLSU - rRNA）基因进行扩增。
结果
通过巢式PCR方法，在60份BAL样本中的10份（16.66%）检测到了[病原体名称未给出]的DNA。
结论
根据目前的研究结果，[病原体名称未给出]的感染率与伊朗其他类似研究报告的感染率相似。
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