紫杉醇对发育中大鼠肝脏血浆蛋白分泌的作用减弱。

Reduced effect of taxol on plasma protein secretion by developing rat liver.

作者信息

Kaufman S S, Tuma D J, Vanderhoof J A

出版信息

Toxicol Appl Pharmacol. 1986 Feb;82(2):233-8. doi: 10.1016/0041-008x(86)90198-5.

Abstract

The effect of taxol, a potent stabilizer of microtubules, on plasma protein secretion by slices prepared from mature and developing rat liver was determined. After 4 hr of incubation, taxol (50 microM) inhibited albumin secretion by only 10% on gestation Day 19, by 16% 4 days after birth, but by 40% in the adult. Inhibition of glycoprotein secretion by taxol was similar to that of albumin secretion at all ages studied. Combined with information obtained from previous investigations, the present study indicates that agents that either inhibit microtubular assembly or that stabilize microtubules both have a reduced capacity to inhibit hepatic protein secretion during development. These findings suggest that a reduced requirement for microtubular participation in protein secretion is present in developing liver.

摘要

研究了强效微管稳定剂紫杉醇对从成年和发育中大鼠肝脏制备的切片血浆蛋白分泌的影响。孵育4小时后，紫杉醇（50微摩尔）在妊娠第19天仅抑制白蛋白分泌10%，出生后4天抑制16%，但在成年大鼠中抑制40%。在所有研究的年龄段，紫杉醇对糖蛋白分泌的抑制作用与白蛋白分泌相似。结合先前研究获得的信息，本研究表明，抑制微管组装或稳定微管的药物在发育过程中抑制肝脏蛋白分泌的能力均降低。这些发现表明，发育中的肝脏对微管参与蛋白分泌的需求降低。