利用拉曼光谱法区分金黄色葡萄球菌和表皮葡萄球菌菌株
Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy.
作者信息
Rebrošová Katarína, Šiler Martin, Samek Ota, Růžička Filip, Bernatová Silvie, Ježek Jan, Zemánek Pavel, Holá Veronika
机构信息
Department of Microbiology, Faculty of Medicine & St Anne's Faculty Hospital, Pekařská 53, Brno 65691, Czech Republic.
ASCR, Institute of Scientific Instruments, Královopolská 147, Brno 61264, Czech Republic.
出版信息
Future Microbiol. 2017 Aug;12:881-890. doi: 10.2217/fmb-2016-0224. Epub 2017 Jul 7.
AIM
Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique.
MATERIALS & METHODS: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy.
DISCUSSION & CONCLUSION: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
目的
拉曼光谱是一种在多个科学领域具有广泛应用的分析方法。我们报告了使用这种快速非侵入性技术区分临床材料中常见的两种重要革兰氏阳性菌——金黄色葡萄球菌和表皮葡萄球菌的可能性。
材料与方法
为此，我们使用拉曼光谱直接对在穆勒-欣顿琼脂平板上生长的菌落中的87株菌株进行了测试，其中金黄色葡萄球菌41株，表皮葡萄球菌46株。
讨论与结论
该方法为即使在大量样本中分离这两种细菌铺平了道路，因此，它有可能用于临床诊断。
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