Raffray Loic, Giry Claude, Thirapathi Yoga, Reboux Anne-Hélène, Jaffar-Bandjee Marie-Christine, Gasque Philippe
Université de La Réunion, CNRS 9192, INSERM U1187, IRD 249, CHU de La Réunion, UMR PIMIT Unité Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, Sainte-Clotilde, La Réunion, France.
Laboratoire de Biologie, CHU La Réunion site Félix Guyon, St Denis, La Réunion, France.
PLoS One. 2017 Jul 7;12(7):e0180474. doi: 10.1371/journal.pone.0180474. eCollection 2017.
Leptospirosis is a multisystemic zoonotic disease with infiltration of visceral organs by Leptospira. The capacity of the vascular endothelium to grant immune cell recruitment and activation in target organs during the disease course remains poorly characterized. We ascertained the levels of expression of several soluble cell adhesion molecules (CAM) notably expressed by endothelial cells in human leptospirosis. We prospectively enrolled 20 hospitalized patients and compared them to 10 healthy controls. Disease severity was defined by one or more organ failures, or death. Plasmatic concentrations of soluble CAM were assessed by multiplex bead assay at the time of patient presentation (M0) and 1 month after hospital discharge. The levels of soluble E-selectin (sCD62E) and soluble intercellular adhesion molecule 1 (sICAM-1, sCD53) were significantly increased in patients compared to controls (p<0.0001) and at 1 month (p<0.0001) with median values at 978 ng/ml (interquartile ranges 787-1164; sCD62E) and 1021 ng/ml (690-1428; sCD53). At M0, Soluble P-selectin level (sCD62P) was found to be decreased with levels at 60 ng/ml (0-631) versus 711 ng/ml (343-1113) for healthy controls (p<0.05). Levels of sICAM-3 (sCD50), sVCAM-1 (vascular cell adhesion molecule, sCD106) and sPECAM-1 (platelet endothelial cell adhesion molecule, sCD31) were not different from healthy subjects at M0. This study shows that two adhesion molecules, shed as soluble forms, are elevated during the acute phase of leptospirosis: E-selectin and s-ICAM1. These molecules may interfere with the process of immune cell recruitment to clear Leptospira at tissue levels.
钩端螺旋体病是一种多系统人畜共患病，钩端螺旋体可浸润内脏器官。在疾病过程中，血管内皮在靶器官中促进免疫细胞募集和激活的能力仍未得到充分表征。我们确定了人类钩端螺旋体病中内皮细胞显著表达的几种可溶性细胞粘附分子（CAM）的表达水平。我们前瞻性地纳入了20名住院患者，并将他们与10名健康对照进行比较。疾病严重程度由一个或多个器官衰竭或死亡定义。在患者就诊时（M0）和出院后1个月，通过多重微珠分析评估可溶性CAM的血浆浓度。与对照组相比，患者体内可溶性E-选择素（sCD62E）和可溶性细胞间粘附分子1（sICAM-1，sCD53）水平显著升高（p<0.0001），在1个月时也显著升高（p<0.0001），中位数分别为978 ng/ml（四分位间距787-1164；sCD62E）和1021 ng/ml（690-1428；sCD53）。在M0时，发现可溶性P-选择素水平（sCD62P）降低，为60 ng/ml（0-631），而健康对照为711 ng/ml（343-1113）（p<0.05）。在M0时，sICAM-3（sCD50）、sVCAM-1（血管细胞粘附分子，sCD106）和sPECAM-1（血小板内皮细胞粘附分子，sCD31）水平与健康受试者无差异。这项研究表明，在钩端螺旋体病急性期，两种以可溶性形式脱落的粘附分子E-选择素和s-ICAM1升高。这些分子可能会干扰免疫细胞在组织水平上募集以清除钩端螺旋体的过程。
