Deficiency of RgpG Causes Major Defects in Cell Division and Biofilm Formation, and Deficiency of LytR-CpsA-Psr Family Proteins Leads to Accumulation of Cell Wall Antigens in Culture Medium by Streptococcus mutans.

is known to possess rhamnose-glucose polysaccharide (RGP), a major cell wall antigen. strains deficient in , encoding the first enzyme of the RGP biosynthesis pathway, were constructed by allelic exchange. The deficiency had no effect on growth rate but caused major defects in cell division and altered cell morphology. Unlike the coccoid wild type, the mutant existed primarily in chains of swollen, "squarish" dividing cells. Deficiency of also causes significant reduction in biofilm formation ( < 0.01). Double and triple mutants with deficiency in and/or , genes coding for the LytR-CpsA-Psr family proteins BrpA and Psr, which were previously shown to play important roles in cell envelope biogenesis, were constructed using the mutant. There were no major differences in growth rates between the wild-type strain and the and double mutants, but the growth rate of the triple mutant was reduced drastically ( < 0.001). Under transmission electron microscopy, both double mutants resembled the mutant, while the triple mutant existed as giant cells with multiple asymmetric septa. When analyzed by immunoblotting, the mutant displayed major reductions in cell wall antigens compared to the wild type, while little or no signal was detected with the double and triple mutants and the and single mutants. These results suggest that RgpG in plays a critical role in cell division and biofilm formation and that BrpA and Psr may be responsible for attachment of cell wall antigens to the cell envelope., a major etiological agent of human dental caries, produces rhamnose-glucose polysaccharide (RGP) as the major cell wall antigen. This study provides direct evidence that deficiency of RgpG, the first enzyme of the RGP biosynthesis pathway, caused major defects in cell division and morphology and reduced biofilm formation by , indicative of a significant role of RGP in cell division and biofilm formation in These results are novel not only in , but also other streptococci that produce RGP. This study also shows that the LytR-CpsA-Psr family proteins BrpA and Psr in are involved in attachment of RGP and probably other cell wall glycopolymers to the peptidoglycan. In addition, the results also suggest that BrpA and Psr may play a direct role in cell division and biofilm formation in This study reveals new potential targets to develop anticaries therapeutics.