变形链球菌细胞壁多糖的生物合成结构与机制
Structure and mechanism of biosynthesis of Streptococcus mutans cell wall polysaccharide.
作者信息
Rush Jeffrey S, Zamakhaeva Svetlana, Murner Nicholas R, Deng Pan, Morris Andrew J, Kenner Cameron W, Black Ian, Heiss Christian, Azadi Parastoo, Korotkov Konstantin V, Widmalm Göran, Korotkova Natalia
机构信息
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA.
Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, USA.
出版信息
Nat Commun. 2025 Jan 22;16(1):954. doi: 10.1038/s41467-025-56205-1.
Streptococcus mutans, the causative agent of human dental caries, expresses a cell wall attached Serotype c-specific Carbohydrate (SCC) that is critical for cell viability. SCC consists of a polyrhamnose backbone of →3)α-Rha(1 → 2)α-Rha(1→ repeats with glucose (Glc) side-chains and glycerol phosphate (GroP) decorations. This study reveals that SCC has one predominant and two more minor Glc modifications. The predominant Glc modification, α-Glc, attached to position 2 of 3-rhamnose, is installed by SccN and SccM glycosyltransferases and is the site of the GroP addition. The minor Glc modifications are β-Glc linked to position 4 of 3-rhamnose installed by SccP and SccQ glycosyltransferases, and α-Glc attached to position 4 of 2-rhamnose installed by SccN working in tandem with an unknown enzyme. Both the major and the minor β-Glc modifications control bacterial morphology, but only the GroP and major Glc modifications are critical for biofilm formation.
变形链球菌是人类龋齿的病原体，它表达一种附着在细胞壁上的血清型c特异性碳水化合物（SCC），这对细胞活力至关重要。SCC由一个→3)α-Rha(1 → 2)α-Rha(1→重复的聚鼠李糖主链组成，带有葡萄糖（Glc）侧链和甘油磷酸（GroP）修饰。本研究表明，SCC有一个主要的和两个次要的Glc修饰。主要的Glc修饰，α-Glc，连接在3-鼠李糖的2位，由SccN和SccM糖基转移酶安装，是GroP添加的位点。次要的Glc修饰是由SccP和SccQ糖基转移酶连接在3-鼠李糖的4位的β-Glc，以及由SccN与一种未知酶协同作用连接在2-鼠李糖的4位的α-Glc。主要和次要的β-Glc修饰都控制细菌形态，但只有GroP和主要的Glc修饰对生物膜形成至关重要。
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