Department of Biochemistry, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University School of Basic Medical Sciences, Wuhan, China; Nursing and Medical School of Technology, Jianghan University, Wuhan, China.
Department of Biochemistry, Gannan Medical University, Ganzhou, China.
Arch Oral Biol. 2017 Nov;83:13-19. doi: 10.1016/j.archoralbio.2017.06.013. Epub 2017 Jun 24.
To investigate the role of parathyroid hormone related protein (PTHrP) in diabetic periodontitis.
After injected with 55mg/kg streptozotocin, diabetic rats were treated subcutaneously with low-dose (40μg/kg, once daily for 5days per week), middle-dose (80μg/kg) or high-dose (160μg/kg) PTHrP(1-34) peptide. Treatment continued for 12 weeks. Changes in periodontal tissues were confirmed by micro-computerized tomography assay and H&E analysis. We used tartrate resistant acid phosphatase (TRAP) staining to identify osteoclast cells. The expression of TNF-α, IL-1β and IL-6 was assessed by immunohistochemistry and Western blot.
Tooth-supporting structure loss was observed in periodontal tissues of diabetic rats. PTHrP (1-34) attenuated alveolar bone loss, especially in the middle-dose and high-dose group. Whereas TNF-α, IL-1β and IL-6 protein levels were increased in the diabetic gingival tissues, PTHrP (1-34) treatment inhibited the increase of IL-1β and IL-6, but had no effect on TNF-α.
Type 1 diabetes increased the susceptibility to periodontal disease. Intermittent administration of PTHrP (1-34) exhibited an inhibitory effect on alveolar bone resorption and the gingival inflammation in periodontal tissues of diabetic rats.
探讨甲状旁腺激素相关蛋白（PTHrP）在糖尿病性牙周炎中的作用。
链脲佐菌素（55mg/kg）注射诱导糖尿病大鼠后，皮下给予低剂量（40μg/kg，每周 5 天，每天 1 次）、中剂量（80μg/kg）或高剂量（160μg/kg）PTHrP（1-34）肽。治疗持续 12 周。通过微计算机断层扫描检测和 H&E 分析确认牙周组织的变化。我们使用抗酒石酸酸性磷酸酶（TRAP）染色来鉴定破骨细胞。通过免疫组化和 Western blot 评估 TNF-α、IL-1β 和 IL-6 的表达。
糖尿病大鼠牙周组织的支持牙齿结构丢失。PTHrP（1-34）减轻了牙槽骨的丢失，尤其是在中剂量和高剂量组。而在糖尿病牙龈组织中，TNF-α、IL-1β 和 IL-6 蛋白水平增加，PTHrP（1-34）治疗抑制了 IL-1β 和 IL-6 的增加，但对 TNF-α没有影响。
1 型糖尿病增加了对牙周病的易感性。间歇性给予 PTHrP（1-34）对糖尿病大鼠牙周组织的牙槽骨吸收和牙龈炎症具有抑制作用。
