Department of Biomedical Engineering, Wrocław University of Science and Technology, pl. Grunwaldzki 13, 50-370 Wrocław, Poland.
Department of Biomedical Engineering, Wrocław University of Science and Technology, pl. Grunwaldzki 13, 50-370 Wrocław, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 5;188:37-49. doi: 10.1016/j.saa.2017.07.001. Epub 2017 Jul 4.
In this work we report the studies of the effect of temperature on skin components, such as lipids, proteins and water. Modifications of lipids structure induced by increasing temperature (from 20 to 90°C) have been studied using ATR-IR (Attenuated Total Reflectance Infrared) spectroscopy, which is a powerful tool for characterization of the molecular structure and properties of tissues, such as skin. Due to the small depth of penetration (0.6-5.6μm), ATR-IR spectroscopy probes only the outermost layer of the skin, i.e. the stratum corneum (SC). The assignment of main spectral features of skin components allows for the determination of phase transitions from the temperature dependencies of band intensities [e.g. ν(CH) and ν(CH)]. The phase transitions were determined by using two methods: the first one was based on the first derivative of the Boltzmann function and the second one employed tangent lines of sigmoidal, aforementioned dependencies. The phase transitions in lipids were correlated with modifications of the structure of water and proteins.
在这项工作中，我们报告了温度对皮肤成分（如脂质、蛋白质和水）的影响研究。使用衰减全反射红外（ATR-IR）光谱研究了温度升高（从 20°C 到 90°C）引起的脂质结构的变化，ATR-IR 光谱是一种用于表征组织（如皮肤）的分子结构和性质的强大工具。由于穿透深度较小（0.6-5.6μm），ATR-IR 光谱仅探测皮肤的最外层，即角质层（SC）。皮肤成分的主要光谱特征的分配允许从带强度的温度依赖性[例如ν（CH）和ν（CH）]来确定相变。使用两种方法确定了相变：第一种方法基于玻尔兹曼函数的一阶导数，第二种方法采用了上述依赖性的正切线。脂质中的相变与水和蛋白质结构的变化相关联。
