壳聚糖量子点用于金黄色葡萄球菌的选择性生物传感。
Selective biosensing of Staphylococcus aureus using chitosan quantum dots.
机构信息
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 804, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 800, Taiwan; Department of Chemistry, Assuit University, Assuit 71515, Egypt.
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 804, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 800, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 80424, Taiwan; Institue of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 5;188:50-56. doi: 10.1016/j.saa.2017.06.047. Epub 2017 Jul 5.
Selective biosensing of Staphylococcus aureus (S. aureus) using chitosan modified quantum dots (CTS@CdS QDs) in the presence of hydrogen peroxide is reported. The method is based on the intrinsic positive catalase activity of S. aureus. CTS@CdS quantum dots provide high dispersion in aqueous media with high fluorescence emission. Staphylococcus aureus causes a selective quenching of the fluorescence emission of CTS@CdS QDs in the presence of HO compared to other pathogens such as Escherichia coli and Pseudomonas aeruginosa. The intrinsic enzymatic character of S. aureus (catalase positive) offers selective and fast biosensing. The present method is highly selective for positive catalase species and requires no expensive reagents such as antibodies, aptamers or microbeads. It could be extended for other species that are positive catalase.
本文报道了壳聚糖修饰的量子点（CTS@CdS QDs）在存在过氧化氢的情况下用于选择性检测金黄色葡萄球菌（S. aureus）的方法。该方法基于金黄色葡萄球菌固有的过氧化氢酶活性。壳聚糖修饰的量子点在水相中具有高荧光发射和高分散性。与其他病原体（如大肠杆菌和铜绿假单胞菌）相比，在存在 HO 的情况下，金黄色葡萄球菌会选择性猝灭 CTS@CdS QDs 的荧光发射。金黄色葡萄球菌（过氧化氢酶阳性）的内在酶特性提供了选择性和快速的生物传感。该方法对阳性过氧化氢酶物种具有高度选择性，且不需要昂贵的试剂，如抗体、适体或微珠。它可以扩展到其他阳性过氧化氢酶的物种。
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