Han En, Yang Yi, He Zheng, Cai Jianrong, Zhang Xinai, Dong Xiaoya
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
Anal Biochem. 2015 Oct 1;486:102-6. doi: 10.1016/j.ab.2015.07.001. Epub 2015 Jul 6.
A sensitive and simple amperometric biosensor for phenols was developed based on the immobilization of tyrosinase into CdS quantum dots/chitosan nanocomposite matrix. The nanocomposite film with porous nanostructure, excellent hydrophilicity and biocompatibility resulted in high enzyme loading, and the tyrosinase (Tyr) immobilized in this novel matrix retained its activity to a large extent. The CdS quantum dots/chitosan nanocomposite film was characterized by scanning electron microscopy and electrochemical impedance spectroscopy, and the parameters of the various experimental variables for the biosensor were optimized. Under the optimal conditions, the designed biosensor displayed a wide linear response to catechol over a concentration range of 1.0×10(-9) to 2.0×10(-5)M with a high sensitivity of 561±9.7mAM(-1) and a low detection limit down to 0.3 nM at a signal-to-noise ratio of 3. The CdS quantum dots/chitosan nanocomposites could provide a novel matrix for enzyme immobilization to promote the development of biosensing and biocatalysis.
基于将酪氨酸酶固定在硫化镉量子点/壳聚糖纳米复合基质中，开发了一种用于检测酚类物质的灵敏且简便的电流型生物传感器。具有多孔纳米结构、优异亲水性和生物相容性的纳米复合膜实现了高酶负载量，并且固定在这种新型基质中的酪氨酸酶（Tyr）在很大程度上保留了其活性。通过扫描电子显微镜和电化学阻抗谱对硫化镉量子点/壳聚糖纳米复合膜进行了表征，并对生物传感器的各种实验变量参数进行了优化。在最佳条件下，所设计的生物传感器对儿茶酚在1.0×10(-9)至2.0×10(-5)M的浓度范围内呈现出宽线性响应，灵敏度高达561±9.7mAM(-1)，在信噪比为3时检测限低至0.3 nM。硫化镉量子点/壳聚糖纳米复合材料可为酶固定化提供一种新型基质，以促进生物传感和生物催化的发展。
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