Laboratório de Neuroproteção e Doenças Neurometabólicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Anexo, Porto Alegre, RS, CEP 90035-003, Brazil.
Neuromolecular Med. 2017 Sep;19(2-3):345-356. doi: 10.1007/s12017-017-8449-7. Epub 2017 Jul 8.
The objective of study was to investigate changes caused by ovariectomy (OVX) on aversive and non-aversive memories, as well as on cytoskeleton phosphorylating system and on vitamin D receptor (VDR) immunocontent in hippocampus. The neuroprotective role of vitamin D was also investigated. Ninety-day-old female Wistar rats were divided into four groups: SHAM, OVX, VITAMIN D and OVX + VITAMIN D; 30 days after the OVX, vitamin D supplementation (500 IU/kg), by gavage, for 30 days was started. Results showed that OVX impaired short-term and long-term recognition, and long-term aversive memories. OVX altered hippocampal cytoskeleton phosphorylating system, evidenced by the hyperphosphorylation of glial fibrillary acidic protein (GFAP), low molecular weight neurofilament subunit (NFL), medium molecular weight neurofilament subunit (NFM) and high molecular weight neurofilament subunit (NFH), and increased the immunocontent of c-Jun N-terminal protein kinases (JNK), Ca/calmodulin-dependent protein kinase II (PKCaMII) and of the sites phosphorylated lysine-serine-proline (KSP) repeats, Ser55 and Ser57. Vitamin D reversed the effects caused by OVX on cytoskeleton in hippocampus, but it was not able to reverse the effects on memory.
本研究旨在探讨卵巢切除术（OVX）对回避性和非回避性记忆、细胞骨架磷酸化系统以及海马体维生素 D 受体（VDR）免疫含量的影响，并研究维生素 D 的神经保护作用。将 90 日龄雌性 Wistar 大鼠分为 4 组：SHAM、OVX、维生素 D 和 OVX+维生素 D；OVX 后 30 天，开始通过灌胃补充维生素 D（500 IU/kg）30 天。结果表明，OVX 损害了短期和长期识别以及长期回避性记忆。OVX 改变了海马体细胞骨架磷酸化系统，表现为神经胶质纤维酸性蛋白（GFAP）、低分子量神经丝亚单位（NFL）、中分子量神经丝亚单位（NFM）和高分子量神经丝亚单位（NFH）过度磷酸化，以及 c-Jun N-末端蛋白激酶（JNK）、Ca/钙调蛋白依赖性蛋白激酶 II（PKCaMII）和磷酸化赖氨酸-丝氨酸-脯氨酸（KSP）重复、丝氨酸 55 和丝氨酸 57 位点的免疫含量增加。维生素 D 逆转了 OVX 对海马体细胞骨架的影响，但未能逆转对记忆的影响。
