分离百日咳博德特氏菌的外膜成分，这些成分可增强b型流感嗜血杆菌荚膜多糖聚核糖基核糖醇磷酸的免疫原性。
Isolation of the outer membrane components of Bordetella pertussis which enhance the immunogenicity of Haemophilus influenzae type b capsular polysaccharide polyribosyl ribitol phosphate.
作者信息
Monji N, Stebbins M R, McCoy D W, Kuo J S
出版信息
Infect Immun. 1986 Mar;51(3):865-71. doi: 10.1128/iai.51.3.865-871.1986.
The outer membrane (OM) component(s) from Bordetella pertussis which potentiated the antigenicity of purified Haemophilus influenzae type b capsular polysaccharide, polyribosyl ribitol phosphate (PRP), has been isolated and partially characterized. The OM was isolated from spheroplasting medium by precipitating at pH 5.0; fractionation was carried out by dissolving the crude OM in Triton X-100 followed by selective precipitation of OM in 50% ethanol. Further purification of OM was accomplished by DEAE-Sepharose 6BCL and Sephacryl S-300 chromatography. The biochemical composition and protein profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of various OM preparations have been examined. Combined vaccines consisting of OM components and PRP were given to young Sprague-Dawley rats, and the serum antibody to PRP was measured by an [3H]PRP binding assay. The purified OM containing mainly a 30,000-dalton protein, but not purified lipopolysaccharide or leukocytosis-promoting toxin, exhibited strong enhancement of PRP immunogenicity.
已从百日咳博德特氏菌中分离出增强纯化的b型流感嗜血杆菌荚膜多糖——多聚核糖基核糖醇磷酸酯（PRP）抗原性的外膜（OM）成分，并对其进行了部分特性鉴定。通过在pH 5.0下沉淀从原生质球形成培养基中分离出OM；将粗制OM溶解于Triton X-100中，随后在50%乙醇中选择性沉淀进行分级分离。通过DEAE-琼脂糖6BCL和Sephacryl S-300色谱法进一步纯化OM。已检测了各种OM制剂的生化组成以及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上的蛋白质图谱。将由OM成分和PRP组成的联合疫苗给予幼年斯普拉格-道利大鼠，并通过[3H]PRP结合试验测定血清中针对PRP的抗体。纯化的OM主要含有一种30,000道尔顿的蛋白质，而不含有纯化的脂多糖或促白细胞增多毒素，其对PRP免疫原性表现出强烈的增强作用。
Zotero 插件