Laboratoire de biologie cellulaire et moléculaire, Faculté de médecine dentaire, Université de Monastir, Rue Avicenne, 5000 Monastir, Tunisia; Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 », Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne, 5000 Monastir, Tunisia.
Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 », Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne, 5000 Monastir, Tunisia.
Eur J Pharmacol. 2017 Oct 5;812:91-96. doi: 10.1016/j.ejphar.2017.07.017. Epub 2017 Jul 6.
Flavonoids are polyphenols frequently consumed in the diet they have been suggested to exert a number of beneficial actions on human health, including anti-inflammatory activity. This study investigated the immunomodulatory effects of two flavonoids, Chrysin and Hesperetin. The effects of flavonoids on B and T cell proliferation were assessed on splenocytes stimulated or not with mitogens. However, their effects on cytotoxic T lymphocyte (CTL) and natural killer (NK) activities were assessed in splenocytes co-incubated with target cells. We report for the first time that both tested flavonoids enhance lymphocyte proliferation at 3.12μM. Chrysin significantly inhibited lipopolysaccharide (LPS) and lectin stimulated splenocyte proliferation. Whereas, hesperetin enhanced LPS and lectin stimulated splenocyte proliferation. In addition, both flavonoids significantly enhance NK cell and CTL activities. Furthermore, our study demonstrated that depending on the concentrations, flavonoid molecules affect macrophage functions by modulating their lysosomal activity and nitric oxide (NO) release, suggesting a potential anti-inflammatory effect. We conclude that flavonoids such as chrysin and hesperetin may be potentially useful for modulating immune cell functions in physiological and pathological conditions and thus a good candidate as food addition component.
类黄酮是一种经常在饮食中摄入的多酚，它们被认为对人类健康有许多有益的作用，包括抗炎活性。本研究调查了两种类黄酮，白杨素和橙皮苷的免疫调节作用。在有丝分裂原刺激或不刺激的脾细胞上评估了类黄酮对 B 和 T 细胞增殖的影响。然而，在与靶细胞共孵育的脾细胞中评估了它们对细胞毒性 T 淋巴细胞 (CTL) 和自然杀伤 (NK) 活性的影响。我们首次报道，这两种测试的类黄酮在 3.12μM 时均增强淋巴细胞增殖。白杨素显著抑制脂多糖 (LPS) 和凝集素刺激的脾细胞增殖。而橙皮苷增强 LPS 和凝集素刺激的脾细胞增殖。此外，这两种类黄酮均显著增强 NK 细胞和 CTL 活性。此外，我们的研究表明，根据浓度的不同，类黄酮分子通过调节其溶酶体活性和一氧化氮 (NO) 释放来影响巨噬细胞功能，提示其具有潜在的抗炎作用。我们得出结论，类黄酮，如白杨素和橙皮苷，可能在生理和病理条件下对调节免疫细胞功能有用，因此是作为食品添加剂的良好候选物。
