Maatouk Mouna, Elgueder Dorra, Mustapha Nadia, Chaaban Hind, Bzéouich Imen Mokdad, Loannou Irina, Kilani Soumaya, Ghoul Mohamed, Ghedira Kamel, Chekir-Ghedira Leila
Unité des Substances Naturells Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Université de Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Cell Stress Chaperones. 2016 Nov;21(6):1101-1109. doi: 10.1007/s12192-016-0734-0. Epub 2016 Sep 13.
Naringenin is one of the most popular flavonoids derived from citrus. It has been reported to be an effective anti-inflammatory compound. Citrus fruit may be used raw, cooked, stewed, or boiled. The present study was conducted to investigate the effect of thermal processes on naringenin in its immunomodulatory and cellular antioxidant activities. The effects of flavonoids on B and T cell proliferation were assessed on splenocytes stimulated or not with mitogens. However, their effects on cytotoxic T lymphocyte (CTL) and natural killer (NK) activities were assessed in splenocytes co-incubated with target cells. The amount of nitric oxide production and the lysosomal enzyme activity were evaluated in vitro on mouse peritoneal macrophages. Cellular antioxidant activity in splenocytes and macrophages was determined by measuring the fluorescence of the dichlorofluorescin (DCF). Our findings revealed that naringenin induces B cell proliferation and enhances NK activity. The highest concentration of native naringenin exhibits a significant proliferation of T cells, induces CTL activity, and inhibits cellular oxidation in macrophages. Conversely, it was observed that when heat-processed, naringenin improves the cellular antioxidant activity in splenocytes, increases the cytotoxic activity of NK cells, and suppresses the cytotoxicity of T cells. However, heat treatment maintains the anti-inflammatory potency of naringenin.
柚皮素是从柑橘类水果中提取的最常见的类黄酮之一。据报道，它是一种有效的抗炎化合物。柑橘类水果可以生食、烹饪、炖煮或煮沸。本研究旨在探讨热加工对柚皮素免疫调节和细胞抗氧化活性的影响。通过对有丝分裂原刺激或未刺激的脾细胞评估类黄酮对B细胞和T细胞增殖的影响。然而，通过与靶细胞共同孵育的脾细胞评估它们对细胞毒性T淋巴细胞（CTL）和自然杀伤（NK）活性的影响。在体外对小鼠腹腔巨噬细胞评估一氧化氮产生量和溶酶体酶活性。通过测量二氯荧光素（DCF）的荧光来测定脾细胞和巨噬细胞中的细胞抗氧化活性。我们的研究结果表明，柚皮素可诱导B细胞增殖并增强NK活性。天然柚皮素的最高浓度表现出T细胞的显著增殖，诱导CTL活性，并抑制巨噬细胞中的细胞氧化。相反，观察到热加工后，柚皮素可提高脾细胞中的细胞抗氧化活性，增加NK细胞的细胞毒性活性，并抑制T细胞的细胞毒性。然而，热处理可保持柚皮素的抗炎效力。
