Kong Lingheng, Wei Ming, Sun Na, Zhu Juanxia, Su Xingli
Institute of Basic Medical Science, School of Basic Medical Sciences, Xi'an Medical College, Xi'an 710021, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2017 Jun 28;42(6):611-616. doi: 10.11817/j.issn.1672-7347.2017.06.002.
To investigate the role of melatonin in calcium overload-induced heart injury. Methods: Thirty-two rats were divided into 4 groups: a control group (Control), a melatonin control group (Mel), a calcium overload group (CaP), and a calcium overload plus melatonin group (Mel+CaP). Isolated Sprague Dawley male rat hearts underwent Langendorff perfusion. Left ventricular developed pressure (LVDP) was calculated to evaluate the myocardial performance. Triphenyltetrazolium chloride staining was used to measure the infarct size of myocardium. Lactate dehydrogenase (LDH) activity in the coronary flow was determined. The expressions of caspase-3 and cytochrome c were determined by Western blot. The pathological morphological changes in myocardial fiber were analyzed by HE staining. Results: Compared with the control group, calcium overload significantly induced an enlarged infarct size (P<0.01), accompanied by the disordered arrangement of myocardial fiber, up-regulation of cytochrome c and caspase-3 (P<0.01), and the increased activity of LDH (P<0.01). These effects were significantly attenuated by 10 μmol/L melatonin (P<0.01). Conclusion: Melatonin can alleviate calcium overload-induced heart injury.
探讨褪黑素在钙超载诱导的心脏损伤中的作用。方法：将32只大鼠分为4组：对照组（Control）、褪黑素对照组（Mel）、钙超载组（CaP）和钙超载加褪黑素组（Mel+CaP）。采用Langendorff灌注法对雄性Sprague Dawley大鼠离体心脏进行灌注。计算左心室发展压（LVDP）以评估心肌功能。采用氯化三苯基四氮唑染色法测量心肌梗死面积。测定冠脉流量中乳酸脱氢酶（LDH）活性。通过蛋白质免疫印迹法检测半胱天冬酶-3（caspase-3）和细胞色素c的表达。采用苏木精-伊红（HE）染色法分析心肌纤维的病理形态学变化。结果：与对照组相比，钙超载显著导致梗死面积增大（P<0.01），伴有心肌纤维排列紊乱、细胞色素c和caspase-3上调（P<0.01）以及LDH活性增加（P<0.01）。10 μmol/L褪黑素可显著减轻这些效应（P<0.01）。结论：褪黑素可减轻钙超载诱导的心脏损伤。
