Zhuo Xiaoqing, Jiang Honglei
MM. Department of Cardiology - Shandong Second Provincial General Hospital (Shandong ENT Hospital) - Jinan, China.
Acta Cir Bras. 2022 May 2;37(2):e370208. doi: 10.1590/acb370208. eCollection 2022.
The present study explored the role of melatonin in cisplatin-induced cardiac injury along with the possible role of brain-derived neurotrophic factor (BDNF) in melatonin-mediated effects.
Wistar rats were administered cisplatin (10 mg/kg), and cardiac injury was assessed by measuring the levels of cardiac troponin (cTnT) and lactate dehydrogenase (LDH-1).The extent of apoptosis was measured by measuring caspase-3 (pro-apoptotic) and Bcl-2 (anti-apoptotic) in hearts. The levels of BDNF, tumour necrosis factor α (TNF-α) and reduced glutathione were measured in heart. Melatonin (5 and 10 mg/kg) was administered for 15 days, and the role of BDNF was identified by co-administering BDNF inhibitor, ANA-12 (0.25 and 0.5 mg/kg).
Melatonin attenuated cTnT and LDH-1 levels along with reduction in caspase-3 and increase in Bcl-2. It also increased cisplatin-induced decrease in BDNF, increase in TNF-α and decrease in reduced glutathione levels. Moreover, ANA-12 abolished the cardioprotective effects, anti-inflammatory and antioxidant effects of melatonin suggesting the role of BDNF in melatonin-mediated effects in cisplatin-induced cardiac injury.
Melatonin is useful in cisplatin-induced cardiac injury, which may be due to an increase in BDNF, decrease in inflammation and increase in antioxidant activities.
本研究探讨褪黑素在顺铂诱导的心脏损伤中的作用，以及脑源性神经营养因子（BDNF）在褪黑素介导的效应中的可能作用。
给Wistar大鼠注射顺铂（10mg/kg），通过测量心肌肌钙蛋白（cTnT）和乳酸脱氢酶（LDH-1）水平评估心脏损伤。通过测量心脏中半胱天冬酶-3（促凋亡）和Bcl-2（抗凋亡）来测定凋亡程度。测量心脏中BDNF、肿瘤坏死因子α（TNF-α）和还原型谷胱甘肽的水平。给予褪黑素（5和10mg/kg）15天，并通过共同给予BDNF抑制剂ANA-12（0.25和0.5mg/kg）来确定BDNF的作用。
褪黑素降低了cTnT和LDH-1水平，同时降低了半胱天冬酶-3水平并增加了Bcl-2水平。它还逆转了顺铂诱导的BDNF降低、TNF-α升高和还原型谷胱甘肽水平降低。此外，ANA-12消除了褪黑素的心脏保护作用、抗炎和抗氧化作用，表明BDNF在褪黑素介导的顺铂诱导的心脏损伤效应中起作用。
褪黑素对顺铂诱导的心脏损伤有效，这可能是由于BDNF增加、炎症减轻和抗氧化活性增强。
