Nomura Chie, Masayama Atsushi, Yamaguchi Mizuka, Sakuma Daisuke, Kajimura Keiji
Osaka Prefectural Institute of Public Health.
Osaka City Institute of Public Health and Environmental Sciences.
Shokuhin Eiseigaku Zasshi. 2017;58(3):132-142. doi: 10.3358/shokueishi.58.132.
In this study, species-specific identification of five toxic mushrooms, Chlorophyllum molybdites, Gymnopilus junonius, Hypholoma fasciculare, Pleurocybella porrigens, and Tricholoma ustale, which have been involved in food-poisoning incidents in Japan, was investigated. Specific primer pairs targeting internal transcribed spacer (ITS) regions were designed for PCR detection. The specific amplicons were obtained from fresh, cooked, and simulated gastric fluid (SGF)-treated samples. No amplicons were detected from other mushrooms with similar morphology. Our method using one-step extraction of mushrooms allows rapid detection within 2.5 hr. It could be utilized for rapid identification or screening of toxic mushrooms.
在本研究中，对曾在日本引发食物中毒事件的五种有毒蘑菇——绿褐裸伞、朱红裸伞、簇生黄韧伞、长根金钱菌和茶褐口蘑进行了物种特异性鉴定。设计了针对内部转录间隔区（ITS）区域的特异性引物对用于PCR检测。从新鲜的、煮熟的以及经模拟胃液（SGF）处理的样本中获得了特异性扩增子。未从其他形态相似的蘑菇中检测到扩增子。我们采用的一步法提取蘑菇样本的方法能够在2.5小时内实现快速检测。该方法可用于有毒蘑菇的快速鉴定或筛查。
