Maeta Kazuhiko, Ochi Tomoya, Tokimoto Keisuke, Shimomura Norihiro, Maekawa Nitaro, Kawaguchi Nobuhisa, Nakaya Makoto, Kitamoto Yutaka, Aimi Tadanori
The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho Minami, Tottori-shi, Tottori 680-8553, Japan.
Appl Environ Microbiol. 2008 May;74(10):3306-9. doi: 10.1128/AEM.02082-07. Epub 2008 Mar 31.
Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.
利用实时聚合酶链式反应(PCR)系统对日本主要的烹饪过的和新鲜的有毒蘑菇进行了物种特异性鉴定。检测到了特异性荧光信号,未检测到非特异性信号。因此,我们成功开发出一种能在1.5小时内鉴定有毒蘑菇的物种特异性检测方法。